An inexpensive "silicone photo device" for transmicroscopic registration of rhythmical movement phenomena.

Microscopica acta Pub Date : 1981-01-01
F Achenbach, U Achenbach, K E Samans, K E Wohlfarth-Bottermann
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Abstract

A highly sensitive electronic unit (called "silicon photo probe") is described, which enables registration of cellular motion phenomena simultaneous with their light microscopic observation. Changes in light intensity caused by movements of the living object are registered by means of a silicon photo diode (silicon blue cell), which can be mounted within the binocular tube of any type of light microscope replacing one of the oculars. Its application during investigations of oscillating contraction activity in Physarum is reported. Advantages and short-comings are discussed with respect to established photometric, tensiometric and infrared registration techniques.

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一种廉价的“有机硅光装置”,用于跨显微镜记录有节奏的运动现象。
描述了一种高灵敏度的电子单元(称为“硅光探针”),它可以在光显微镜观察的同时记录细胞运动现象。活体物体的运动引起的光强度的变化是通过硅光电二极管(硅蓝色电池)来记录的,硅光电二极管可以安装在任何类型的光学显微镜的双筒管中,以取代一台望远镜。报道了其在绒泡菌振荡收缩活性研究中的应用。讨论了现有的光度法、张力法和红外配准技术的优缺点。
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