[Comparative study of five different methods for grouping beta-haemolytic streptococci (author's transl)].

H J Lögering
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Abstract

161 strains of beta-haemolytic streptococci, that had been isolated from patients' material, and 7 reference strains were group]ed with the techniques of precipitation, direct immunofluorescence, co-agglutination (Phadebact Streptococcus Test), latex agglutination (Streptex), and biochemical tests (Api-Strep). From the reference strains one group G strain was incorrectly identified with the Api-Strep. The direct immunofluorescence test yielded a result in agreement with the precipitation method in 94% of the freshly isolated strains. Co-agglutination and latex agglutination gave corresponding results with the precipitation method in 99%, taking into consideration that the first method doesn't apply to groups D and F. The biochemical determination with Api-Strep gave correct results with all strains of groups A and B, but did not do so with some group G and most of the group C streptococcal strains. Therefore especially co-agglutination and latex agglutination can be recommended for routine diagnostic purposes as reliable, rapid, and simple methods.

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[五种不同的乙型溶血链球菌分类方法的比较研究[作者简介]。
采用沉淀法、直接免疫荧光法、共凝集法(Phadebact Streptococcus Test)、乳胶凝集法(Streptex)和生化法(Api-Strep)对从患者标本中分离得到的161株乙型溶血链球菌和7株参比菌株进行分组。从参考菌株中,1组G菌株被错误地识别为Api-Strep。在94%的新分离菌株中,直接免疫荧光试验的结果与沉淀法一致。考虑到第一种方法不适用于D组和f组,共凝集法和乳胶凝集法在99%范围内给出了相应的结果。Api-Strep法对A组和B组所有菌株的生化测定结果正确,但对部分G组和大部分C组链球菌的生化测定结果不正确。因此,特别是共凝集和乳胶凝集作为可靠、快速和简单的常规诊断方法,可以推荐用于常规诊断目的。
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