{"title":"Lysis of cell nuclei on perforated electron microscopic supports.","authors":"J Erenpreisa","doi":"","DOIUrl":null,"url":null,"abstract":"<p><p>The technique of gentle nuclear lysis for electron microscopic observation is suggested, which comprises adsorption of isolated nuclei on a positively charged pricked supporting film and sequential short-timed installation of the support with its back onto the filter paper moistened by lysing solution. The advantage of the method is slow, gradient-like, and to some extent regulated treatment allowing to visualize nuclear structures in their integrity.</p>","PeriodicalId":76158,"journal":{"name":"Microscopica acta","volume":"85 4","pages":"369-80"},"PeriodicalIF":0.0000,"publicationDate":"1982-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Microscopica acta","FirstCategoryId":"1085","ListUrlMain":"","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 0
Abstract
The technique of gentle nuclear lysis for electron microscopic observation is suggested, which comprises adsorption of isolated nuclei on a positively charged pricked supporting film and sequential short-timed installation of the support with its back onto the filter paper moistened by lysing solution. The advantage of the method is slow, gradient-like, and to some extent regulated treatment allowing to visualize nuclear structures in their integrity.
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