{"title":"Indirect immunoperoxidase technique for the assay of antibodies against Mycoplasma gallisepticum and M. synoviae in chicken serum.","authors":"Y Imada, I Nonomura, K Furuta","doi":"","DOIUrl":null,"url":null,"abstract":"<p><p>The indirect immunoperoxidase technique (IIP) was applied to the assay of antibodies against Mycoplasma gallisepticum and M. synoviae in chicken serum by using colonies grown on the agar plate as antigen. the sensitivity and the specificity of IIP were evaluated by the use of sera from experimentally infected, filed, and SPF Chickens. As compared with tube agglutination and hemagglutination-inhibition tests, IIP was highly effective and specific for detecting antibodies against M. gallisepticum and M. synoviae.</p>","PeriodicalId":76197,"journal":{"name":"National Institute of Animal Health quarterly","volume":"22 1","pages":"16-22"},"PeriodicalIF":0.0000,"publicationDate":"1982-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"National Institute of Animal Health quarterly","FirstCategoryId":"1085","ListUrlMain":"","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 0
Abstract
The indirect immunoperoxidase technique (IIP) was applied to the assay of antibodies against Mycoplasma gallisepticum and M. synoviae in chicken serum by using colonies grown on the agar plate as antigen. the sensitivity and the specificity of IIP were evaluated by the use of sera from experimentally infected, filed, and SPF Chickens. As compared with tube agglutination and hemagglutination-inhibition tests, IIP was highly effective and specific for detecting antibodies against M. gallisepticum and M. synoviae.
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