{"title":"Some characteristics of nystatin-resistant sterol mutants of Candida albicans.","authors":"M Pesti, S Paku, E K Novák","doi":"","DOIUrl":null,"url":null,"abstract":"<p><p>Various stable, auxotrophic and nystatin-resistant sterol mutants of Candida albicans were isolated after nitrosoguanidine treatment. Sterol mutants were divided into groups on the basis of the ultraviolet spectra and thin-layer chromatographic patterns of their nonsaponifiable sterol extracts. They were further characterized by their conductometrically measured nystatin-induced ion release. These sterol mutants displayed a decreased growth yield and an increased cell volume. On media containing 0.01% of the carbon sources, most of them could assimilate glycerol, alpha-methyl-D-glucoside, DL-lactic acid, L-sorbose, L-arabinose and ribitol only to a significantly reduced extent, or not at all. It is presumed that these properties result from the altered sterol composition of the plasma membrane.</p>","PeriodicalId":75387,"journal":{"name":"Acta microbiologica Academiae Scientiarum Hungaricae","volume":"29 1","pages":"55-66"},"PeriodicalIF":0.0000,"publicationDate":"1982-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Acta microbiologica Academiae Scientiarum Hungaricae","FirstCategoryId":"1085","ListUrlMain":"","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 0
Abstract
Various stable, auxotrophic and nystatin-resistant sterol mutants of Candida albicans were isolated after nitrosoguanidine treatment. Sterol mutants were divided into groups on the basis of the ultraviolet spectra and thin-layer chromatographic patterns of their nonsaponifiable sterol extracts. They were further characterized by their conductometrically measured nystatin-induced ion release. These sterol mutants displayed a decreased growth yield and an increased cell volume. On media containing 0.01% of the carbon sources, most of them could assimilate glycerol, alpha-methyl-D-glucoside, DL-lactic acid, L-sorbose, L-arabinose and ribitol only to a significantly reduced extent, or not at all. It is presumed that these properties result from the altered sterol composition of the plasma membrane.
经亚硝基胍处理后，分离出多种稳定的、营养不良的和耐制氨抑素的白色念珠菌甾醇突变体。根据其不皂化甾醇提取物的紫外光谱和薄层色谱图对甾醇突变体进行了分类。它们通过电导法测量的制氨抑素诱导的离子释放进一步表征。这些甾醇突变体表现出生长产量下降和细胞体积增加。在碳源含量为0.01%的培养基上，大多数菌体对甘油、α -甲基- d -葡萄糖苷、dl -乳酸、l-山梨糖、l-阿拉伯糖和利比醇的同化作用仅显著降低或完全不吸收。据推测，这些特性是由质膜的固醇组成改变引起的。
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