{"title":"Alloantigen-mediated adherence of rat hepatocytes to antibody-coated polystyrene dishes.","authors":"J M Hunt, M T Buckley, B A Laishes","doi":"10.3109/08820138209050736","DOIUrl":null,"url":null,"abstract":"<p><p>Rat alloantigens expressed on normal hepatocytes have been utilized in a cell \"panning\" procedure to mediate the haplotype-specific adherence of collagenase-isolated hepatocytes to antibody-coated polystyrene dishes. A polyvalent F344 anti-WF alloantiserum was prepared by immunizing F344 rats with WF tissue. The alloantibody IgG purified from the alloantiserum, when reacted with either WF or (WF X F344)F1 hybrid rat hepatocytes, caused the adherence of these hepatocytes to bacteriological-grade polystyrene dishes coated non-specifically with a xeno-antibody, rabbit anti-rat IgG. Similarly-treated hepatocytes of the F344 strain, which lack WF alloantigenic determinants, failed to be adsorbed above background level to the coated dishes.</p>","PeriodicalId":13417,"journal":{"name":"Immunological communications","volume":"11 5","pages":"377-86"},"PeriodicalIF":0.0000,"publicationDate":"1982-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.3109/08820138209050736","citationCount":"4","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Immunological communications","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.3109/08820138209050736","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 4
Abstract
Rat alloantigens expressed on normal hepatocytes have been utilized in a cell "panning" procedure to mediate the haplotype-specific adherence of collagenase-isolated hepatocytes to antibody-coated polystyrene dishes. A polyvalent F344 anti-WF alloantiserum was prepared by immunizing F344 rats with WF tissue. The alloantibody IgG purified from the alloantiserum, when reacted with either WF or (WF X F344)F1 hybrid rat hepatocytes, caused the adherence of these hepatocytes to bacteriological-grade polystyrene dishes coated non-specifically with a xeno-antibody, rabbit anti-rat IgG. Similarly-treated hepatocytes of the F344 strain, which lack WF alloantigenic determinants, failed to be adsorbed above background level to the coated dishes.
在正常肝细胞上表达的大鼠同种抗原已被用于细胞“淘洗”过程，以介导胶原酶分离的肝细胞对抗体包被的聚苯乙烯培养皿的单倍型特异性粘附。用WF组织免疫F344大鼠制备多价F344抗WF同种抗血清。从同种异体抗血清中纯化的同种异体抗体IgG，当与WF或(WF X F344)F1杂交大鼠肝细胞反应时，导致这些肝细胞粘附在细菌级聚苯乙烯培养皿上，培养皿非特异性地涂有异种抗体兔抗大鼠IgG。同样处理的F344菌株的肝细胞，缺乏WF同种抗原决定因子，不能被高于背景水平的吸附到包被培养皿上。
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