Urea synthesis by perfused rat liver in experimental uremia.

G Perez, B Rietberg, B Owens, T Parker, H Obaya, E Schiff
{"title":"Urea synthesis by perfused rat liver in experimental uremia.","authors":"G Perez,&nbsp;B Rietberg,&nbsp;B Owens,&nbsp;T Parker,&nbsp;H Obaya,&nbsp;E Schiff","doi":"10.1159/000176358","DOIUrl":null,"url":null,"abstract":"<p><p>Urea synthesis was examined in experimental uremia using the isolated perfused rat liver in order to assure strict control of substrate (NH4Cl) presented to the liver. Acute uremia was created in female Sprague-Dawley rats by bilateral nephrectomy (n = 7) 48 h prior to studies. Chronic uremia (8--14 weeks) was produced by right nephrectomy and segmental infarction of the left kidney in 7 rats. At infusion rates of NH4Cl (8.3 mumol/min) which resulted in prehepatic perfusate ammonia levels approximately twice the previously described Km value, livers of chronically uremic rats had slightly higher rates of urea production than controls (controls: 0.41 +/- 0.03; chronic uremia: 0.54 +/- 0.04 mumol/min/g of wet liver weight; p less than 0.02). In acute uremia, urea production was higher (0.66 +/- 0.05 mumol/min/g) than in sham-operated rats (0.59 +/- 0.05 mumol/min/g) but the differences did not achieve statistical significance. Simultaneously performed taurocholate transport studies did not reveal significant functional differences between the livers of uremic and control animals. The data suggest that urea production by livers of uremic rats is increased when compared to that of control animals.</p>","PeriodicalId":19333,"journal":{"name":"Nutrition and metabolism","volume":"24 6","pages":"409-16"},"PeriodicalIF":0.0000,"publicationDate":"1980-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1159/000176358","citationCount":"5","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Nutrition and metabolism","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1159/000176358","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 5

Abstract

Urea synthesis was examined in experimental uremia using the isolated perfused rat liver in order to assure strict control of substrate (NH4Cl) presented to the liver. Acute uremia was created in female Sprague-Dawley rats by bilateral nephrectomy (n = 7) 48 h prior to studies. Chronic uremia (8--14 weeks) was produced by right nephrectomy and segmental infarction of the left kidney in 7 rats. At infusion rates of NH4Cl (8.3 mumol/min) which resulted in prehepatic perfusate ammonia levels approximately twice the previously described Km value, livers of chronically uremic rats had slightly higher rates of urea production than controls (controls: 0.41 +/- 0.03; chronic uremia: 0.54 +/- 0.04 mumol/min/g of wet liver weight; p less than 0.02). In acute uremia, urea production was higher (0.66 +/- 0.05 mumol/min/g) than in sham-operated rats (0.59 +/- 0.05 mumol/min/g) but the differences did not achieve statistical significance. Simultaneously performed taurocholate transport studies did not reveal significant functional differences between the livers of uremic and control animals. The data suggest that urea production by livers of uremic rats is increased when compared to that of control animals.

查看原文
分享 分享
微信好友 朋友圈 QQ好友 复制链接
本刊更多论文
实验性尿毒症大鼠肝脏灌注合成尿素。
为了严格控制给肝的底物(NH4Cl),采用离体灌注大鼠肝脏对实验性尿毒症尿素合成进行了检测。研究前48小时,雌性Sprague-Dawley大鼠双侧肾切除术(n = 7)造成急性尿毒症。7只大鼠右肾切除术和左肾节段性梗死引起慢性尿毒症(8 ~ 14周)。在NH4Cl输注速率(8.3 μ mol/min)导致肝前灌注氨水平约为先前描述的Km值的两倍时,慢性尿毒症大鼠的肝脏尿素生成率略高于对照组(对照组:0.41 +/- 0.03;慢性尿毒症:湿肝重0.54±0.04 μ mol/min/g;P < 0.02)。急性尿毒症大鼠尿素产量(0.66 +/- 0.05 mumol/min/g)高于假手术大鼠(0.59 +/- 0.05 mumol/min/g),但差异无统计学意义。同时进行的牛磺胆酸盐转运研究没有发现尿毒症动物和对照动物肝脏之间的显著功能差异。数据表明,与对照动物相比,尿毒症大鼠肝脏的尿素产量增加。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
求助全文
约1分钟内获得全文 去求助
来源期刊
自引率
0.00%
发文量
0
期刊最新文献
"Post-coronary" rehabilitation, body composition, and recurrent infarction. An analysis of data from the Ontario Exercise-Heart Collaborative Study. Effects of undernutrition on nucleic acid synthesis in neuronal and glial cells from different regions of developing rat brain. Oral and parenteral nutrition with L-Valine: mode of action. Studies on the extent of dietary fiber intake in West Germany. Na+,K+-ATPase in developing rat brain during undernutrition.
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
现在去查看 取消
×
提示
确定
0
微信
客服QQ
Book学术公众号 扫码关注我们
反馈
×
意见反馈
请填写您的意见或建议
请填写您的手机或邮箱
已复制链接
已复制链接
快去分享给好友吧!
我知道了
×
扫码分享
扫码分享
Book学术官方微信
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术
文献互助 智能选刊 最新文献 互助须知 联系我们:info@booksci.cn
Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。
Copyright © 2023 Book学术 All rights reserved.
ghs 京公网安备 11010802042870号 京ICP备2023020795号-1