A differential labeling model for determining the number of catalytically essential carboxyl groups in fumarase

Robert C. Seid Jr. , Thomas P. Sakmar
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Abstract

We have developed a mathematical model of the nonideal case in which enzymatic activity changes may also result from modification of non-essential groups. As an illustration of this method, the number of essential carboxyl groups in pig heart fumarase (l-malate hydro-lyase, EC 4.2.1.2) was determined by the differential labeling technique. Enzymatic activity was related to the number of modified carboxyl groups according to the model and the results were compatible with the existence of two essential carboxyl groups in fumarase.

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确定延胡索酶中催化必需羧基数量的微分标记模型
我们已经开发了一个非理想情况下的数学模型,其中酶活性的变化也可能是由非必需基团的修饰引起的。以猪心脏延胡索酸酶(l-苹果酸水解酶,EC 4.2.1.2)中必需羧基的数量为例,采用差异标记技术进行了测定。根据模型,酶活性与修饰羧基的数量有关,结果与富马酸酶中两个必需羧基的存在相一致。
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