{"title":"Determination of brain regional normetanephrine levels by liquid chromatography with electrochemical detection (LC-EC)","authors":"Andrew S. Chiu , Damodar D. Godse, Jerry J. Warsh","doi":"10.1016/0364-7722(81)90046-1","DOIUrl":null,"url":null,"abstract":"<div><p></p><ul><li><span>1.</span><span><p>1. A liquid chromatography-electrochemical detection (LC-EC) assay for brain normetanephrine (NMN) has been developed which also allows concurrent determination of brain norepinephrine (NE), dopamine (DA) and serotonin (5-HT) concentrations.</p></span></li><li><span>2.</span><span><p>2. Tissue samples were prepurified by cation exchange chromatography and the amines then separated by reversed-phase liquid chromatography on an octylsilane stationary phase.</p></span></li><li><span>3.</span><span><p>3. This method permits measurement of at least 200 pg of the above amines in brain.</p></span></li><li><span>4.</span><span><p>4. The concentrations of NMN in rat and mouse brain regions were found to be in the range of 0.115–0.435 ng/mg protein.</p></span></li></ul></div>","PeriodicalId":20801,"journal":{"name":"Progress in neuro-psychopharmacology","volume":null,"pages":null},"PeriodicalIF":0.0000,"publicationDate":"1981-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0364-7722(81)90046-1","citationCount":"4","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Progress in neuro-psychopharmacology","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/0364772281900461","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 4
Abstract
1.
1. A liquid chromatography-electrochemical detection (LC-EC) assay for brain normetanephrine (NMN) has been developed which also allows concurrent determination of brain norepinephrine (NE), dopamine (DA) and serotonin (5-HT) concentrations.
2.
2. Tissue samples were prepurified by cation exchange chromatography and the amines then separated by reversed-phase liquid chromatography on an octylsilane stationary phase.
3.
3. This method permits measurement of at least 200 pg of the above amines in brain.
4.
4. The concentrations of NMN in rat and mouse brain regions were found to be in the range of 0.115–0.435 ng/mg protein.
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