[Analysis of the biological effect of city smog extract. VI. Comparative investigations on the effect of city smog extracts and their fractions on DNA synthesis of hamster kidney and embryonic cells and kidney cells of the primate Cercopithecus aethiops (author's transl)].

Zentralblatt fur Bakteriologie. 1. Abt. Originale B, Hygiene, Krankenhaushygiene, Betriebshygiene, praventive Medizin Pub Date : 1980-02-01

G Krampitz, N Seemayer

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Abstract

We analyzed the effect of two samples of city smog extracts and their fractions from Duisburg on the rate of DNA synthesis of mammalian cell cultures. - We employed tissue cultures of kidney and embryonic cells from the Syrian golden hamster (Cricetus aureatus) and kidney cells from the African green monkey (Cercopithecus aethiops). The DNA synthesis was determined autoradiographically after incubation with 3H-thymidine. We found that both city smog extracts exerted a dose-dependent suppression of cellular DNA synthesis. Monkey kidney cells were more sensitive than rodent cells. Analysis of single fractions indicate that the inhibition of DNA synthesis is the result of combined effects of all fractions. At present time it is not possible to correlate the toxic effect of the complete extract special to a single fraction or compound group.

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城市雾霾提取物的生物效应分析。六、城市雾霾提取物及其组分对仓鼠肾脏、胚胎细胞及灵长类猴尾猿肾脏细胞DNA合成影响的比较研究[作者译文]。

我们分析了杜伊斯堡两种城市烟雾提取物及其组分对哺乳动物细胞培养DNA合成率的影响。-我们采用了叙利亚金仓鼠(Cricetus aureatus)的肾脏和胚胎细胞以及非洲绿猴(Cercopithecus aethiops)的肾脏细胞的组织培养。用3h -胸腺嘧啶孵育后用放射自显影法测定DNA合成。我们发现两种城市烟雾提取物对细胞DNA合成都有剂量依赖性的抑制。猴肾细胞比啮齿动物细胞更敏感。单组分分析表明，对DNA合成的抑制作用是各组分共同作用的结果。目前还不可能将全提取物的毒性作用与单个部分或化合物组联系起来。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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Zentralblatt fur Bakteriologie. 1. Abt. Originale B, Hygiene, Krankenhaushygiene, Betriebshygiene, praventive Medizin

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