Large-scale purification of prothoracicotropic hormone of the silkworm (Bombyx mori).

Scientia Sinica Pub Date : 1980-08-01

H Nagasawa, F Guo, X C Zhong, B Y Xia, Z S Wang, X J Qui, D Y Wei, E I Chen, J Z Wang, A Suzuki, A Isogai, Y Hori, S Tamura, H Ishizaki

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Abstract

Prothoracicotropic hormone has been successfully extracted from as many as 2,800,000 silkworm male adult heads and purified partially through an 8-step procedure. Approximately 7 microgram of the crude preparation ("crude PTTH") shows activity in the debrained Samia pupa test. A further 4-step chromatographic purification of the "crude PTTH" derived from 100,000 Bombyx heads yields 3.4 mg of purified preparation ("highly purified PTTH"), of which 7 ng causes adult development in a debrained Samia pupa.

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家蚕促胸激素的大规模纯化。

促胸激素已成功地从多达280万只成年蚕雄蚕头中提取，并经过8个步骤进行部分纯化。大约7微克的粗制剂(“粗PTTH”)在去脑的Samia蛹试验中显示出活性。从100,000家蚕头中提取的“粗PTTH”进一步进行4步色谱纯化，得到3.4毫克纯化制剂(“高纯度PTTH”)，其中7毫克可在去脑的Samia蛹中引起成虫发育。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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Scientia Sinica

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