Biochemical attempt to characterize thirteen cichlid species by their muscular parvalbumins.

B Focant, P Laleye, P Vandewalle
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引用次数: 14

Abstract

This paper shows the usefulness of polyacrylamide gel electrophoresis (PAGE) separation of a few well-characterized muscle proteins, the parvalbumins, in the systematic study of 13 cichlid species. The separation of the isoforms of these abundant, quite species-specific fish parvalbumins is fast, easy, and requires but small quantities of muscle material. Used alone, this technique suggests hypotheses for species classification. In conjunction with morphological analysis, it makes it possible to confirm or invalidate doubts about the determination.

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用13种慈鲷的肌肉小蛋白进行生物化学鉴定。
本文展示了聚丙烯酰胺凝胶电泳(PAGE)分离几种具有良好特征的肌肉蛋白,即小蛋白,在13种慈鲷的系统研究中的作用。分离这些丰富的、非常具有物种特异性的鱼小白蛋白的同种异构体是快速、容易的,而且只需要少量的肌肉材料。单独使用,这种技术提出了物种分类的假设。结合形态分析,可以证实或否定对测定的怀疑。
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