Generation of monoclonal antibodies that distinguish between mesotheliomas and other tumor of the lung.

K Narumi, F A Chen, R B Bankert, H Takita
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Abstract

The accurate diagnosis of mesothelioma remains difficult despite advances of diagnostic technique. And specific monoclonal antibody (McAb) against mesothelioma have not been reported. In an attempt to develop mesothelioma specific McAb(s), spleen cells from a mouse immunized with isolated tumor cells were fused to a drug resistant mouse myeloma cell lines. Over 200 hybridomas were assayed for their preferential reactivity with mesothelioma cell lines or mesothelioma tumor biopsy tissues. Two monoclonal antibodies 2A3 and 4E1 were identified that bound 6/7 of the mesotheliomas, tested, but did not bind to the majority, 11/13 (for 2A3) and 12/13 (for 4E1), of other lung tumor types. Based upon western blot analysis of one and two-dimensional gels and upon the distribution pattern of the antibody recognized molecule in mesotheliomas and non-mesothelioma lung tumors, 2A3 binds to the cell adhesion molecule CD44. While the specificity of 4E1 has not yet been unequivocally established it appears to recognize a variant form of the CD44 molecule.

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产生区分间皮瘤和其他肺肿瘤的单克隆抗体。
尽管诊断技术不断进步,但间皮瘤的准确诊断仍然很困难。而针对间皮瘤的特异性单克隆抗体(McAb)尚未见报道。在开发间皮瘤特异性单克隆抗体的尝试中,用分离的肿瘤细胞免疫小鼠的脾脏细胞与耐药小鼠骨髓瘤细胞系融合。研究了200多个杂交瘤对间皮瘤细胞系或间皮瘤活检组织的优先反应性。鉴定出两种单克隆抗体2A3和4E1结合了6/7的间皮瘤,但不结合大多数其他肺肿瘤类型,11/13 (2A3)和12/13 (4E1)。基于一维和二维凝胶的western blot分析以及抗体识别分子在间皮瘤和非间皮瘤肺肿瘤中的分布模式,2A3与细胞粘附分子CD44结合。虽然4E1的特异性尚未明确确定,但它似乎识别CD44分子的一种变体形式。
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