Christopher Molloy, Maxwell G. Shepherd, Patrick A. Sullivan
{"title":"Differential Extraction of N-Acetylglucosaminidase and Trehalase from the Cell Envelope of Candida albicans","authors":"Christopher Molloy, Maxwell G. Shepherd, Patrick A. Sullivan","doi":"10.1006/emyc.1995.1022","DOIUrl":null,"url":null,"abstract":"<div><p>Molloy, C., Shepherd, M. G., and Sullivan, P. A. 1995. Differential extraction of <em>N</em>-acetylglucosaminidase and trehalase from the cell envelope of <em>Candida albicans. Experimental Mycology</em> 19, 178-185. Dithiothreitol (DTT) extraction of <em>N</em>-acetylglucosaminidase and trehalase from intact <em>Candida albicans</em> ATCC 10261 cells was monitored as an index of cell envelope porosity during <em>N</em>-acetylglucosamine-induced morphogenesis. Trehalase, which is secreted into the cell envelope during starvation and bud-formation, displayed similar extraction kinetics in starved, germ tube-forming, and bud-forming cells, indicating that the mother cell wall remains largely unchanged during morphogenic outgrowth and that the porosity of bud and mother cell walls is similar. <em>N</em>-acetylglucosaminidase, which is secreted specifically during morphogenesis, was released eightfold more rapidly from germ tube-forming than bud-forming cells, reflecting major differences in porosity between bud and germ tube. In addition, by assaying DTT extracts and extracted cell residues, it was found that the total extracellular <em>N</em> -acetylglucosaminidase activity increased 2- to 2.5-fold during DTT treatment. Thus, DTT unmasks a cryptic form of <em>N</em>-acetylglucosaminidase. The cryptic activity was associated with the cell wall fraction.</p></div>","PeriodicalId":12110,"journal":{"name":"Experimental Mycology","volume":"19 3","pages":"Pages 178-185"},"PeriodicalIF":0.0000,"publicationDate":"1995-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1006/emyc.1995.1022","citationCount":"11","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Experimental Mycology","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0147597585710225","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 11
Abstract
Molloy, C., Shepherd, M. G., and Sullivan, P. A. 1995. Differential extraction of N-acetylglucosaminidase and trehalase from the cell envelope of Candida albicans. Experimental Mycology 19, 178-185. Dithiothreitol (DTT) extraction of N-acetylglucosaminidase and trehalase from intact Candida albicans ATCC 10261 cells was monitored as an index of cell envelope porosity during N-acetylglucosamine-induced morphogenesis. Trehalase, which is secreted into the cell envelope during starvation and bud-formation, displayed similar extraction kinetics in starved, germ tube-forming, and bud-forming cells, indicating that the mother cell wall remains largely unchanged during morphogenic outgrowth and that the porosity of bud and mother cell walls is similar. N-acetylglucosaminidase, which is secreted specifically during morphogenesis, was released eightfold more rapidly from germ tube-forming than bud-forming cells, reflecting major differences in porosity between bud and germ tube. In addition, by assaying DTT extracts and extracted cell residues, it was found that the total extracellular N -acetylglucosaminidase activity increased 2- to 2.5-fold during DTT treatment. Thus, DTT unmasks a cryptic form of N-acetylglucosaminidase. The cryptic activity was associated with the cell wall fraction.