Phylogenetic Ordinal Placement Based on rDNA Sequences of the Freshwater Genera Ophioceras and Pseudohalonectria

Weidong Chen, Carol A. Shearer, John Klopp
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引用次数: 15

Abstract

Chen, W., Shearer, C. A., and Klopp, J. 1995. Phylogenetic ordinal placement based on rDNA sequences of the freshwater genera Ophioceras and Pseudohalonectria. Experimental Mycology 19, 191-201, The ordinal placement of two closely related freshwater genera, Ophioceras and Pseudohalonectria , was assessed by using phylogenetic analysis of morphological characters, partial sequences of the large subunit ribosomal DNA and restriction site variations in the internal transcribed spacer (ITS). The two genera have some morphological features that are used to define taxa in both the Sordariales and Diaporthales, and, hence, their phylogenetic relationships are unclear. Equally weighted analyses of thirty-eight morphological characters produced unresolved phylogenetic trees and unequivocal conclusions could not be drawn based on the morphological data. The polymcrase chain reaction-amplified ITS region was variable in length between the two genera and restriction sites in the ITS region were determined. Analysis of variation in restriction sites in the ITS region placed Ophioceras and Pseudohalonectria in one clade with taxa sampled from Sordariales. About 350 basepairs of DNA sequence from the 5′ end of the large subunit rDNA were also determined. In phylogenetic analysis of the sequence data with Hypocrea lutea and Nectria cinnabarina as outgroups, Ophioceras and Pseudohalonectria showed a closer relationship to Neurospora crassa , Schizothecium sp., and Sordaria fimicola of the Sordariales than to Cryphonectria parasitica and Endothia gyrosa of the Diaporthales.

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基于淡水蛇麻属和假盐碱属rDNA序列的系统发育顺序定位
陈,W, Shearer, C. A.和Klopp, J. 1995。基于淡水蛇麻属和假盐藻属rDNA序列的系统发育顺序定位。通过形态学特征、大亚基核糖体DNA部分序列和内部转录间隔区(ITS)限制性位点变异的系统发育分析,对两个亲缘关系较近的淡水属Ophioceras和Pseudohalonectria的序位进行了评价。这两个属具有一些形态特征,用于定义Sordariales和Diaporthales的分类群,因此,它们的系统发育关系尚不清楚。对38个形态特征的同等加权分析产生了未解决的系统发育树,并且无法根据形态数据得出明确的结论。聚合酶链反应扩增的ITS区长度在两属之间是可变的,并确定了ITS区的限制性位点。ITS区限定位点的变异分析将蛇孔目和假盐孔目与Sordariales的分类群归为一个分支。从大亚基rDNA的5 '端也确定了大约350个碱基对的DNA序列。以黄下体和朱砂Nectria cinnabarina为外群的序列数据进行系统发育分析,发现蛇孔虫和假盐孔虫与Sordariales的Neurospora crassa、Schizothecium sp.和Sordaria finimicola的亲缘关系比与Diaporthales的Cryphonectria parasitica和Endothia gyrosa的亲缘关系更近。
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