Intraspecific Variation within Populations of Fusarium oxysporum Based on RFLP Analysis of the Intergenic Spacer Region of the rDNA

Diane J Appel, Thomas R Gordon
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引用次数: 155

Abstract

Appel, D. J., and Gordon, T. R. 1995. Intraspecific variation within populations of Fusarium oxysporum based on RFLP analysis of the intergenic spacer region of the rDNA. Experimental Mycology 19, 120-128. Fifty-six isolates of Fusarium oxysporum, including F. oxysporum f. sp. melonis and nonpathogenic strains, were chosen from a larger collection to represent diversity in vegetative compatibility groups (VCGs), mitochondrial DNA (mtDNA) haplotype, geographic distribution, and virulence. Using PCR, a 2.6-kb fragment including the intergenic spacer (IGS) region of the ribosomal DNA was amplified from each isolate. The enzymes EcoRI, Sau 3A, Cfo1, and Ava1I, cut this fragment differentially, revealing 5, 6, 6, and 7 patterns, respectively. Among the 56 isolates, a total of 13 unique IGS haplotypes was identified. Among most F. o. melonis isolates. IGS haplotype correlated with VCG and mtDNA haplotype, but did not differentiate among races. However, a race 1 isolate found in VCG 0131 shared virulence, mtDNA, and IGS haplotypes characteristic of VCG 0134; this isolate may represent a conversion in VCG from 0134 to 0131. Four nonpathogens shared the pathogen vegetative compatibility phenotypes. One race 1,2 isolate associated with VCG 0134 shared both IGS haplotype and VCG with a nonpathogen, but these isolates did not share the same mtDNA haplotype. Another nonpathogenic isolate shared mtDNA and IGS haplotypes with pathogen group 0131 and may simply be an avirulent mutant of a pathogenic strain. For the other two nonpathogenic isolates, vegetative compatibility indicated a close relationship to the pathogen, but differences in both mtDNA and IGS haplotype suggest otherwise. Overall, the IGS haplotype was more variable among the nonpathogenic F. oxysporum VCGs among which 12 of the 13 IGS haplotypes were found. Nonpathogenic isolates that shared a common mtDNA haplotype, but were associated with different VCGs, often had different IGS haplotypes.

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基于rDNA基因间隔区RFLP分析的尖孢镰刀菌种群内种内变异
Appel, D. J.和Gordon, T. R. 1995。基于rDNA基因间隔区RFLP分析的尖孢镰刀菌种群内种内变异。真菌学学报,19(2):393 - 398。从大量收集的56株尖孢镰刀菌(Fusarium oxysporum F. sp. melonis)和非致病性菌株中选取尖孢镰刀菌(Fusarium oxysporum F. sp. melonis),分析其营养相容性群体(VCGs)、线粒体DNA (mtDNA)单倍型、地理分布和毒力的多样性。利用PCR技术,从每个分离物中扩增出包含核糖体DNA基因间间隔区(IGS)的2.6 kb片段。酶EcoRI、Sau 3A、Cfo1和Ava1I切割该片段的方式不同,分别显示出5、6、6和7种模式。在56株分离株中,共鉴定出13个独特的IGS单倍型。在大多数甜瓜f.o.分离株中。IGS单倍型与VCG和mtDNA单倍型相关,但在种族间不存在差异。然而,在VCG 0131中发现的1小种分离物具有VCG 0134的毒力、mtDNA和IGS单倍型特征;该分离物可能代表VCG从0134到0131的转换。四种非病原体均具有病原体营养相容性表型。与VCG 0134相关的1、2小种分离株具有IGS单倍型和与非病原体的VCG单倍型,但这些分离株不具有相同的mtDNA单倍型。另一种非致病性分离物与病原体群0131共享mtDNA和IGS单倍型,可能只是致病性菌株的无毒突变体。对于其他两个非致病性分离株,营养相容性表明与病原体关系密切,但mtDNA和IGS单倍型的差异表明并非如此。总体而言,IGS单倍型在非致病性尖孢镰刀菌VCGs中变异较多,其中13个IGS单倍型中有12个存在。具有共同mtDNA单倍型但与不同vcg相关的非致病性分离株通常具有不同的IGS单倍型。
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