Molecular Cloning and Sequence of the Cytoplasmic Ribosomal Protein S15a Gene from Agaricus bisporus

Peter J Schaap, Piet W.J de Groot, Yvonne Müller, Leo J.L.D van Griensven, Jaap Visser
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引用次数: 12

Abstract

Schaap, P. J., de Groot, P. W. J., Müller, Y., van Griensven, L. J. L. D., and Visser, J. 1995. Molecular cloning and sequence of the cytoplasmic ribosomal protein S15a gene from Agaricus bisporus. Experimental Mycology 19, 160-162. We have isolated an Agaricus bisporus cDNA which encodes an open reading frame of 130 amino acids. A comparison with the Genbank database shows that the deduced amino acid sequence of this open reading frame is highly homologous to the small subunit ribosomal proteins S15a of Brassica napus and Drosophila melanogaster and to the small subunit ribosomal proteins S24 of Strongylocentrotus purpuratus and Saccharomyces cerevisiae .

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双孢蘑菇细胞质核糖体蛋白S15a基因的克隆与序列分析
Schaap, P. J, de Groot, P. W. J, m ller, Y., van Griensven, L. J. L. D.和Visser, J. 1995。双孢蘑菇细胞质核糖体蛋白S15a基因的克隆及序列分析。实验真菌学,19,160-162。我们分离到了一个双孢蘑菇cDNA,该cDNA编码130个氨基酸的开放阅读框。与Genbank数据库比对表明,该开放阅读框的氨基酸序列与甘蓝型油菜(Brassica napus)和黑腹果蝇(Drosophila melanogaster)的小亚基核糖体蛋白S15a高度同源,与purpuratus Strongylocentrotus purpuratus和酿酒酵母(Saccharomyces cerevisiae)的小亚基核糖体蛋白S24高度同源。
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