Development of gene probes for the specific identification of Streptococcus uberis and Streptococcus parauberis based upon large subunit rRNA gene sequences.

The Journal of applied bacteriology Pub Date : 1993-05-01
N M Harland, J A Leigh, M D Collins
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引用次数: 0

Abstract

23S ribosomal RNa (rRNA) gene sequences of Streptococcus uberis and Strep. parauberis were determined by direct polymerase chain reaction (PCR) sequencing. Oligonucleotide probes specific for Strep. uberis and Strep. parauberis were designed from variable regions of the 23S rRNA gene sequence data. Molecular hybridizations with PCR-amplified rRNA gene targets provided a precise and reliable means of differentiating Strep. uberis and Strep. parauberis from each other and from other streptococcal species.

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基于大亚基rRNA基因序列特异性鉴定ubercoccus and parauberis的基因探针的研制。
兔链球菌和链球菌23S核糖体RNa (rRNA)基因序列。采用直接聚合酶链反应(PCR)测序法测定副黄酮类化合物。链球菌特异性寡核苷酸探针。uberis和链球菌。从23S rRNA基因序列数据的可变区域设计parauberis。分子杂交与pcr扩增的rRNA基因靶点提供了一种精确和可靠的方法来区分链球菌。uberis和链球菌。从其他链球菌种类和其他链球菌种类中分离。
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