Antibacterial effect of protamine assayed by impedimetry.

C Johansen, T Gill, L Gram
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引用次数: 65

Abstract

Impedimetric measurements were used to assay the antibacterial effect of protamine. A good linear correlation between the impedance detection time and the initial cell counts was obtained (r = 0.99, n = 2). As basic peptides may cause clumping of cells, this correlation curve was used when estimating the cell number after protamine treatment, rather than colony counts. Protamine from salmon killed growing Gram-positive bacteria and significantly inhibited growth of Gram-negative bacteria in Tryptone Soy Broth (TSB) at 25 degrees C. In general Gram-positive bacteria were more sensitive to protamine than Gram-negative bacteria; the minimum inhibitory concentrations (MIC) determined for Gram-positive strains varied from 20 to 1000 micrograms ml-1 and for Gram-negative strains from 500 micrograms ml-1 to more than 4000 micrograms ml-1. The effect of protamine on non-growing Listeria monocytogenes Scott A suspended in buffer was not lethal as was the effect on growing cells; however, protamine (50-500 micrograms ml-1) killed the Gram-negative fish spoilage bacteria Shewanella putrefaciens when the live cells were suspended in buffer.

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障碍法测定鱼精蛋白的抑菌效果。
采用阻抗法测定鱼精蛋白的抑菌效果。阻抗检测时间与初始细胞计数之间具有良好的线性相关性(r = 0.99, n = 2)。由于碱性肽可能导致细胞结块,因此在估计鱼精蛋白处理后的细胞数量时使用该相关曲线,而不是使用菌落计数。在25℃的色氨酸豆汤(TSB)中,鲑鱼鱼精蛋白能杀死生长中的革兰氏阳性菌,显著抑制革兰氏阴性菌的生长,革兰氏阳性菌对鱼精蛋白的敏感性高于革兰氏阴性菌;革兰氏阳性菌株测定的最低抑菌浓度(MIC)在20 ~ 1000微克ml-1之间,革兰氏阴性菌株测定的最低抑菌浓度在500 ~ 4000微克ml-1之间。鱼精蛋白对悬浮在缓冲液中的非生长单核增生李斯特菌Scott A的杀伤作用不像对生长细胞的杀伤作用那样致命;然而,当活细胞悬浮在缓冲液中时,鱼精蛋白(50-500微克ml-1)可以杀死革兰氏阴性的鱼腐败细菌谢氏菌。
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