Characterization of a natural hemagglutinin in the serum of a freshwater crab Parathelphusa hydrodromus (Herbst).

Archives internationales de physiologie, de biochimie et de biophysique Pub Date : 1994-09-01 DOI:10.3109/13813459409003941

M Nalini, P Mullainadhan, M Arumugam

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引用次数: 11

Abstract

A naturally occurring hemagglutinin (HA) was detected in the serum of the freshwater crab Parathelphusa hydrodromus using mammalian erythrocytes (RBC) as indicator cells. The serum gave the highest HA titer with rabbit RBC. In cross adsorption tests, this RBC type completely adsorbed all HA activities from serum. An analysis of the physico-chemical properties of HA showed it to be specifically dependent on the presence of Ca2+ for its activity, irreversibly sensitive to EDTA, stable between pH 7.5 and 10.0, and heat-labile. Further studies demonstrated that the HA is proteinaceous as it was precipitable by conventional deproteinizing agents, and susceptible to the action of proteases and 2-mercaptoethanol. HA-inhibition assays performed with 44 carbohydrates revealed that the serum HA was specific for non-reducing terminal glucose with alpha 1-2 glycosidic linkage. Thus this agglutinin appears to be unique among all the known crustacean agglutinins.

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一种淡水蟹(Parathelphusa hydrodromus, Herbst)血清中天然血凝素的表征。

以哺乳动物红细胞(RBC)为指示细胞，在淡水蟹(Parathelphusa hydrodromus)血清中检测到一种天然存在的血凝素(HA)。兔红细胞血凝素滴度最高。在交叉吸附试验中，这种红细胞完全吸附了血清中所有的HA活性。对HA理化性质的分析表明，它的活性特别依赖于Ca2+的存在，对EDTA不可逆敏感，在pH 7.5和10.0之间稳定，并且热不稳定。进一步的研究表明，透明质酸是蛋白性的，因为它可以被传统的去蛋白剂沉淀，并且对蛋白酶和2-巯基乙醇的作用敏感。对44种碳水化合物进行的HA抑制试验表明，血清HA对α - 1-2糖苷连锁的非还原性末端葡萄糖具有特异性。因此，这种凝集素在所有已知的甲壳类凝集素中似乎是独一无二的。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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Archives internationales de physiologie, de biochimie et de biophysique

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