Two kinds of lateral flow immunoassays based on multifunctional magnetic prussian blue nanoenzyme and colloidal gold for the detection of 38 β-agonists in swine urine and pork

IF 8.5 1区农林科学 Q1 CHEMISTRY, APPLIED Food Chemistry Pub Date : 2023-08-15 DOI:10.1016/j.foodchem.2023.135897

Jinxuan Liang , Zhiwei Liu , Yalin Fang , Xing Shen , Zhenlin Xu , Hongtao Lei , Xianhui Huang , Xiangmei Li

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引用次数: 6

Abstract

Herein, novel multifunctional magnetic prussian blue nanoenzymes (MPBNs) and colloidal gold (CG) were synthesized and used to develop two kinds of lateral flow immunoassays (LFIAs) for the detection of 38 β-agonists. Since MPBNs has a unique three-in-one function of colorimetric magnetic catalytic activities, the signal intensity and coupling ratio are 2 and 8-fold higher than that of the CG. The cut-off values of the CG-LFIA and MPBNs-LFIA for swine urine and pork are 5/5 and 0.3/0.5 μg/kg, the limits of detection are 0.19/0.29 and 0.02/0.03 μg/kg, respectively. The sensitivity of MPBNs-LFIA is 10-fold higher than that of CG-LFIA, and up to 200-fold higher than that of the reported LFIAs. The recoveries of the LFIAs are 80.0%–116.7%, with coefficients of variation of 1.4%–14.3%. Our study proved that the MPBNs have more advantages than CG, and can offer a promising signal label for ultrasensitive immunoassay techniques.

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基于多功能磁普鲁士蓝纳米酶和胶体金的两种侧流免疫分析法检测猪尿和猪肉中38种β-激动剂

本文合成了新型多功能磁性普鲁士蓝纳米酶(mpbn)和胶体金(CG)，并利用它们建立了两种检测38种β-激动剂的侧流免疫分析法(LFIAs)。由于mpbn具有独特的比色磁催化活性三合一功能，其信号强度和耦合比分别比CG高2倍和8倍。CG-LFIA和MPBNs-LFIA对猪尿和猪肉的检测截止值分别为5/5和0.3/0.5 μg/kg，检出限分别为0.19/0.29和0.02/0.03 μg/kg。MPBNs-LFIA的灵敏度比CG-LFIA高10倍，比已报道的lfia高200倍。加样回收率为80.0% ~ 116.7%，变异系数为1.4% ~ 14.3%。我们的研究证明mpbn比CG有更多的优势，可以为超灵敏免疫分析技术提供一个有前途的信号标记。

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来源期刊

Food Chemistry 工程技术-食品科技

CiteScore

16.30

自引率

10.20%

发文量

3130

审稿时长

122 days

期刊介绍： Food Chemistry publishes original research papers dealing with the advancement of the chemistry and biochemistry of foods or the analytical methods/ approach used. All papers should focus on the novelty of the research carried out.