The effect of streptozotocin on the function of fetal porcine and rat pancreatic (pro-)islets.

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引用次数: 6

Abstract

Streptozotocin (STZ) is a broad spectrum antibiotic with anti-tumor and diabetogenic properties. Although STZ has been studied for many years, the exact mechanism of its diabetogenic action has not yet been fully elucidated. The present study investigated the effect of STZ on both fetal porcine proislets (FPP) and fetal rat islets (FRI) in an attempt to elucidate the diabetogenic effect of STZ on fetal pancreatic beta cells. This study demonstrates that after in vitro exposure of both FPP and FRI for 30 min to 2.2 mM and 4.4 mM STZ, respectively, FPP showed microscopically an intact structure, a spherical shape and a translucent color, while, in contrast, most FRI were disrupted and showed a slight white color with dark centers. Based on these data, we first transplanted FPP and FRI beneath the renal capsules of nude mice. Three to four weeks later, a single dosage of streptozotocin (180 mg/kg) was intravenously administered. Six of the seven nude mice pretransplanted with FPP became diabetic (blood glucose, BG, 308.08 +/- 33.62 mg/dl) within 2-5 days and then gradually achieved normoglycemia 51.56 +/- 7.71 days after STZ injection. After removal of the grafts, all of the six diabetic mice with normoglycemia returned to hyperglycemia (BG > 300 mg/dl). In contrast, all of the five nude mice pretransplanted with FRI persistently maintained hyperglycemia (BG > 300 mg/dl) and died 5 +/- 0.84 days after STZ injection.(ABSTRACT TRUNCATED AT 250 WORDS)

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链脲佐菌素对胎猪和大鼠胰腺(前)胰岛功能的影响。
链脲佐菌素(STZ)是一种具有抗肿瘤和致糖尿病特性的广谱抗生素。虽然STZ已被研究多年,但其致糖尿病作用的确切机制尚未完全阐明。本研究研究了STZ对胎猪proislets (FPP)和胎大鼠胰岛(FRI)的影响,试图阐明STZ对胎胰腺β细胞的致糖尿病作用。本研究表明,FPP和FRI分别在2.2 mM和4.4 mM STZ下体外暴露30min后,FPP在显微镜下结构完整,呈球形,颜色半透明,而大多数FRI则被破坏,呈微白色,中心偏暗。基于这些数据,我们首先将FPP和FRI移植到裸鼠肾囊下。3 ~ 4周后,静脉给予单剂量链脲佐菌素(180 mg/kg)。FPP预移植的7只裸鼠中,有6只在2-5天内出现糖尿病(血糖,BG, 308.08 +/- 33.62 mg/dl),注射STZ后逐渐达到正常血糖51.56 +/- 7.71天。移除移植物后,6只血糖正常的糖尿病小鼠均恢复到高血糖状态(BG > 300 mg/dl)。相比之下,5只预移植FRI的裸鼠在注射STZ后5 +/- 0.84 d均持续维持高血糖(BG > 300 mg/dl),死亡。(摘要删节250字)
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