Immunohistochemical studies on specificity and affinity of the BB5-antibody against human parathyroid tissue.

U Joseph, H G Seesko, K Neumann, W Cance, S Wells, M Rothmund
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引用次数: 1

Abstract

Localisation of parathyroid tissue in hyperparathyroidism may be difficult with standard methods. Immunoscintigraphy, using radiolabeled antibodies against epitopes of human parathyroid cells, could be a promising alternative. Therefore, we studied the necessary preconditions, whether the so-called BB5-antibody, directed against parathyroid cell membranes possesses sufficient specificity and affinity to be employed in immunoscintigraphy. Specificity was tested immunohistochemically with APAAP-staining of 39 different human tissues. Additionally, an immunoscore-based quantitative comparison was performed to test the affinity of BB5-antibody for normal and pathologic parathyroid tissue. Specificity was proven by the fact that of all 39 tested tissue-types only the parathyroid tissue was BB5-positive. Normal parathyroid tissue showed a significantly higher affinity to the BB5-antibody than pathologic parathyroid tissue (p < 0.03). However, all tissue samples from primary or secondary hyperparathyroidism exhibited sufficient staining. We conclude that the BB5-antibody fulfills the necessary conditions to be tried for immunoscintigraphical localisation of the parathyroid glands.

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免疫组化研究bb5抗体对人甲状旁腺组织的特异性和亲和力。
甲状旁腺功能亢进症的甲状旁腺组织定位可能很难用标准方法。利用放射性标记抗体对抗人甲状旁腺细胞表位的免疫扫描技术可能是一种很有前途的替代方法。因此,我们研究了针对甲状旁腺细胞膜的所谓bb5抗体是否具有足够的特异性和亲和力以用于免疫扫描的必要前提。用apap染色对39种不同的人体组织进行免疫组织化学特异性检测。此外,采用基于免疫评分的定量比较来检测bb5抗体对正常和病理甲状旁腺组织的亲和力。在所有39种测试组织类型中,只有甲状旁腺组织呈bb5阳性,这一事实证明了特异性。正常甲状旁腺组织对bb5抗体的亲和力显著高于病理甲状旁腺组织(p < 0.03)。然而,所有来自原发性或继发性甲状旁腺功能亢进的组织样本都显示出足够的染色。我们的结论是,bb5抗体满足了甲状旁腺免疫成像定位的必要条件。
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