Comparison of the effects of glycerol, mannitol, and urea on ischemic hippocampal damage in gerbils.

Acta neurochirurgica. Supplementum Pub Date : 1994-01-01 DOI:10.1007/978-3-7091-9334-1_86

K Otsubo, Y Katayama, F Kashiwagi, H Muramatsu, A Terashi

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引用次数: 4

Abstract

The effects of glycerol and mannitol, as well as urea, on delayed neuronal death (DND) in the gerbil hippocampus were investigated. 20% solution of glycerol, mannitol and urea were prepared, and 6.5 ml/kg of each agent, or saline, was administered to male Mongolian gerbils intraperitoneally 30 min before ischemia. The animals were subjected to transient forebrain ischemia for 5 min. Seven days after the ischemic insult, the brains were fixed and stained for histopathological analysis. The number of normal neurons (neuronal density, ND) in a 1 mm linear length of hippocampal CA1 region was counted. ND of sham-operated group (n = 6) was 275.3 +/- 16.7 (mean +/- SD). ND in the saline-treated group (n = 6) was 14.8 +/- 5.0. ND of groups treated with glycerol (n = 6), mannitol (n = 6) and urea (n = 4) was 68.2 +/- 56.7 (p < 0.01), 52.8 +/- 54.4 (p < 0.01) and 12.0 +/- 2.5 (NS), respectively. The present study demonstrates that glycerol and mannitol have some protective effects against DND in the gerbil hippocampus, whereas urea has no effect.

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甘油、甘露醇和尿素对沙鼠缺血性海马损伤影响的比较。

研究了甘油、甘露醇以及尿素对沙鼠海马延迟性神经元死亡(DND)的影响。配制20%甘油、甘露醇和尿素溶液，分别于缺血前30 min给雄性沙鼠腹腔注射6.5 ml/kg或生理盐水。大鼠短暂性前脑缺血5 min，缺血后7 d，固定脑，染色进行组织病理学分析。计数海马CA1区1 mm线长内正常神经元数(神经元密度，ND)。假手术组(n = 6) ND为275.3 +/- 16.7(平均+/- SD)。盐水处理组(n = 6)的ND为14.8±5.0。甘油组(n = 6)、甘露醇组(n = 6)和尿素组(n = 4)的ND分别为68.2 +/- 56.7 (p < 0.01)、52.8 +/- 54.4 (p < 0.01)和12.0 +/- 2.5 (NS)。本研究表明，甘油和甘露醇对沙鼠海马DND有一定的保护作用，而尿素没有作用。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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Acta neurochirurgica. Supplementum

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