Degradation and utilization of forage hemicellulose by rumen bacteria, singly in coculture or added sequentially.

M Fondevila, B A Dehority
{"title":"Degradation and utilization of forage hemicellulose by rumen bacteria, singly in coculture or added sequentially.","authors":"M Fondevila,&nbsp;B A Dehority","doi":"10.1111/j.1365-2672.1994.tb04399.x","DOIUrl":null,"url":null,"abstract":"<p><p>Procedures for sequential addition experiments were developed to study the mechanisms involved in the synergistic and inhibitory interactions observed in forage hemicellulose digestion by rumen bacterial cocultures. One organism was allowed to ferment a forage substrate, the culture tube was sterilized and then inoculated with a second organism. No differences were found in the extent of degradation or utilization between fermentations sterilized by oxidation or heat, and based on ease of handling, heat was used in all subsequent experiments. Studies were conducted with Fibrobacter succinogenes A3c, Ruminococcus flavefaciens B34b and Prevotella ruminicola H2b, singly and in all possible combinations. Results from the sequential addition studies substantiated earlier suggestions that the increase observed in hemicellulose utilization results from initial solubilization of the hemicellulose from the forage by the non-utilizer and subsequent utilization of subsequent utilization of this solubilized polysaccharide by the utilizing, but non-degrading organism.</p>","PeriodicalId":22599,"journal":{"name":"The Journal of applied bacteriology","volume":"77 5","pages":"541-8"},"PeriodicalIF":0.0000,"publicationDate":"1994-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1111/j.1365-2672.1994.tb04399.x","citationCount":"34","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"The Journal of applied bacteriology","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1111/j.1365-2672.1994.tb04399.x","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 34

Abstract

Procedures for sequential addition experiments were developed to study the mechanisms involved in the synergistic and inhibitory interactions observed in forage hemicellulose digestion by rumen bacterial cocultures. One organism was allowed to ferment a forage substrate, the culture tube was sterilized and then inoculated with a second organism. No differences were found in the extent of degradation or utilization between fermentations sterilized by oxidation or heat, and based on ease of handling, heat was used in all subsequent experiments. Studies were conducted with Fibrobacter succinogenes A3c, Ruminococcus flavefaciens B34b and Prevotella ruminicola H2b, singly and in all possible combinations. Results from the sequential addition studies substantiated earlier suggestions that the increase observed in hemicellulose utilization results from initial solubilization of the hemicellulose from the forage by the non-utilizer and subsequent utilization of subsequent utilization of this solubilized polysaccharide by the utilizing, but non-degrading organism.

查看原文
分享 分享
微信好友 朋友圈 QQ好友 复制链接
本刊更多论文
瘤胃细菌对饲料半纤维素的降解和利用,可单独共培养或依次添加。
为了研究在瘤胃细菌共培养中观察到的饲料半纤维素消化的协同和抑制相互作用的机制,建立了顺序添加实验程序。一种微生物在草料基质上发酵,对培养管进行灭菌,然后用另一种微生物接种。氧化灭菌和加热灭菌的发酵在降解和利用程度上没有差异,基于易于处理,在随后的所有实验中都使用了加热。对琥珀酸原纤维杆菌A3c、黄瘤球菌B34b和反刍杆菌普雷沃菌H2b进行了单独和所有可能的组合研究。顺序添加研究的结果证实了先前的建议,即半纤维素利用率的增加是由于非利用者对饲料中的半纤维素的初始溶解作用以及利用但不降解的生物对这种被溶解的多糖的后续利用。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
求助全文
约1分钟内获得全文 去求助
来源期刊
自引率
0.00%
发文量
0
期刊最新文献
Yersinia enterocolitica. Isolation, partial characterization and mode of action of acidocin J1229, a bacteriocin produced by Lactobacillus acidophilus JCM 1229. Note: isolation, characterization and epidemiology of Yersinia enterocolitica from humans and animals. Application of antimicrobial-producing lactic acid bacteria to control pathogens in ready-to-use vegetables. The effect of chlorhexidine on defined, mixed culture oral biofilms grown in a novel model system.
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
现在去查看 取消
×
提示
确定
0
微信
客服QQ
Book学术公众号 扫码关注我们
反馈
×
意见反馈
请填写您的意见或建议
请填写您的手机或邮箱
已复制链接
已复制链接
快去分享给好友吧!
我知道了
×
扫码分享
扫码分享
Book学术官方微信
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术
文献互助 智能选刊 最新文献 互助须知 联系我们:info@booksci.cn
Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。
Copyright © 2023 Book学术 All rights reserved.
ghs 京公网安备 11010802042870号 京ICP备2023020795号-1