Isolation of Legionella from water samples using various culture methods.

J M Kusnetsov, H R Jousimies-Somer, A I Nevalainen, P J Martikainen
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引用次数: 20

Abstract

The efficacy of a non-selective medium and two selective media were compared for the isolation of legionellas from water samples. The effect of acid wash treatment for decontamination of the water samples on the isolation frequency of legionellas was also studied. The 236 samples were taken from cooling, humidifying and drinking water systems; 21% were legionella-positive when inoculated directly on modified Wadowsky-Yee (MWY) medium and 26% were positive when concentrated (x 200) before cultivation on MWY or CCVC media. Inoculation on MWY medium after concentration followed by decontamination by the acid-wash technique gave the highest isolation frequency (31%). The lowest frequency (8%) was found with the non-selective BCYE alpha medium. An isolation frequency of 28% was achieved with the BCYE alpha medium after concentration and acid-wash treatment of the samples. Forty per cent of the samples were positive for legionellas when the results from all the culture methods were combined. Not all the legionella-positive samples were identified by a single culture method. Ninety-three of the 95 positive samples were detected with the two best combinations of three culture methods. The best culture method for detecting legionellas depended on the source of the water sample. Some water quality characteristics, like temperature and organic matter content, affected the isolation frequency of Legionella spp.

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不同培养方法从水样中分离军团菌。
比较了非选择培养基和两种选择培养基分离水样军团菌的效果。研究了酸洗净化水样对军团菌分离频率的影响。这236个样本取自冷却、加湿和饮用水系统;在改良Wadowsky-Yee (MWY)培养基上直接接种时军团菌阳性21%,在MWY或CCVC培养基上培养前浓缩(× 200)时阳性26%。浓缩后在MWY培养基上接种,再用酸洗技术去污,分离率最高(31%)。非选择性BCYE α培养基的发生率最低(8%)。对样品进行浓缩和酸洗处理后,BCYE α培养基的分离率为28%。当综合所有培养方法的结果时,40%的样品对军团菌呈阳性。并非所有军团菌阳性样本都能通过单一培养方法进行鉴定。95份阳性样品中有93份为3种培养方法的2种最佳组合。军团菌的最佳培养方法取决于水样的来源。水温、有机质含量等水质特征影响军团菌的分离频率。
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