Change of isoenzyme pattern during long-term polyxenic cultivation of Entamoeba histolytica.

N Vrchotová, O Ditrich, M Giboda
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引用次数: 2

Abstract

Isoenzymes of phosphoglucomutase and hexokinase were repeatedly evaluated using starch gel electrophoresis in polyxenic cultures of Entamoeba histolytica. In two out of 18 strains spontaneous changes of isoenzyme patterns were recorded. While originally they were categorized into virulent group of zymodemes, following isoenzyme analysis classified them as non-virulent. The relation between virulence and isoenzyme pattern is questionable.

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溶组织内阿米巴长期多种培养过程中同工酶谱的变化。
用淀粉凝胶电泳法对溶组织内阿米巴多源培养的磷酸葡萄糖糖脲酶和己糖激酶同工酶进行了反复测定。在18株菌株中,有2株记录了同工酶模式的自发变化。虽然最初它们被归类为有毒酶组,但在同工酶分析后将它们归类为无毒酶组。毒力与同工酶模式之间的关系尚存疑问。
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