The development and assessment of DNA and oligonucleotide probes for the specific detection of Bacillus anthracis.

R A Hutson, C J Duggleby, J R Lowe, R J Manchee, P C Turnbull
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引用次数: 27

Abstract

Two DNA probes and a number of oligonucleotide probes were designed from the virulence factor genes of Bacillus anthracis. These probes were tested for specificity against 52 B. anthracis strains and 233 Bacillus strains encompassing 23 other species. A rapid slot blotting technique was used for screening the large numbers of isolates involved. All probes tested appeared to be specific for B. anthracis under high stringency conditions. These probes could differentiate between virulent and avirulent strains. The probes were also applied to the detection of B. anthracis in routine environmental and clinical samples. A non-radioactive hybridization and detection system based on digoxigenin-11-dUTP was developed.

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炭疽芽孢杆菌DNA和寡核苷酸特异性检测探针的研制与评价。
从炭疽芽孢杆菌毒力因子基因中设计了两个DNA探针和多个寡核苷酸探针。对52株炭疽芽孢杆菌和233株芽孢杆菌进行了特异性检测。采用快速槽印迹技术对大量分离株进行了筛选。在高严格条件下,所有检测的探针似乎对炭疽芽孢杆菌具有特异性。这些探针可以区分毒株和无毒毒株。该探针还可用于常规环境和临床样品中炭疽芽胞杆菌的检测。研制了一种以地高辛-11- dutp为基础的非放射性杂交检测系统。
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