Immunoreactive hepatocyte growth factor is present in tissue extracts from human breast cancer but not in conditioned medium of human breast cancer cell lines.

J Yamashita, M Ogawa, T Beppu
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Abstract

Hepatocyte growth factor (HGF) is a novel mitogen for mature hepatocytes. In the present study, we have measured immunoreactive (ir)-HGF concentration in tumor extracts of 82 primary human breast cancers using an enzyme-linked immunosorbent assay (ELISA). Ir-HGF was detectable in all tissue extracts, the concentration ranging from 1.4 to 306.5 ng/100 mg protein (median value: 11.2 ng/100 mg protein). Correlation analyses between ir-HGF concentration and clinicopathological factors showed that the ir-HGF level was significantly higher in tumors with sizes of more than 5.0 cm compared with those less than 5.0 cm. In contrast, no detectable amount of ir-HGF was secreted into culture medium of two breast cancer cell lines, MCF-7 and ZR-75-1, suggesting that the cancer cell itself has no ability to produce ir-HGF.

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免疫反应性肝细胞生长因子存在于人乳腺癌组织提取物中,但不存在于人乳腺癌细胞系的条件培养基中。
肝细胞生长因子(HGF)是一种用于成熟肝细胞的新型丝裂原。在本研究中,我们使用酶联免疫吸附试验(ELISA)测量了82例原发性人乳腺癌肿瘤提取物中的免疫反应性(ir)-HGF浓度。在所有组织提取物中均检测到Ir-HGF,浓度范围为1.4 ~ 306.5 ng/100 mg蛋白(中位数为11.2 ng/100 mg蛋白)。ir-HGF浓度与临床病理因素的相关性分析显示,大于5.0 cm的肿瘤中ir-HGF水平明显高于小于5.0 cm的肿瘤。相比之下,MCF-7和ZR-75-1两种乳腺癌细胞系的培养基中没有分泌到可检测到的ir-HGF,这表明癌细胞本身没有能力产生ir-HGF。
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