Effect of protein deficiency on luteinizing hormone releasing hormone (LHRH), gonadotropin releasing hormone associated peptide (GAP) and luteinizing hormone (LH) immunocytochemistry in the hypothalamus and pituitary gland of prepubertal ewes.

J Polkowska, F Przekop
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引用次数: 16

Abstract

Growing female lambs were fed diets containing 14.2% (standard) or 8.1% (protein restricted) of proteins to determine their effects on puberty and luteinizing hormone releasing hormone (LHRH), gonadotropin hormone associated peptide (GAP), luteinizing hormone (LH) hormonal system. At the end of the experiment (30-34 weeks of age), hypothalamic LHRH, GAP and pituitary LH were analysed by immunocytochemical methods using specific antibodies. Plasma LH were determined by radioimmunoassay at 21 weeks of age. It was found that lowering of the dietary proteins content decreased the concentration of basal plasma LH significantly in lambs of 21 weeks of age. None of the sheep of this group reached sexual maturity at the same time as the animals of the standard group. However, immunoreactive (ir) LHRH neuronal system of protein restricted lambs was normally developed: Numerous irLHRH perikarya, dense network of axons and abundant material stored in the nerve terminals were well visualized in the typical sites of the preoptico-septal area, hypothalamus and the median eminence (ME). Gonadotropin associated peptide (GAP) of the LHRH precursor was present in the same populations of neurons that contained LHRH in the sheep brain. The proportion of pituitary LH-cells was three fold higher in pituitaries of the nutritionally restricted group. They displayed hypertrophy and very strong immunoreaction. These results show that protein deficiency in diets of growing female sheep delays their puberty but does not impair the synthesis and processing of LHRH in the brain neurons and synthesis of LH in pituitary cells.(ABSTRACT TRUNCATED AT 250 WORDS)

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蛋白质缺乏对青春期前母羊下丘脑和垂体促黄体生成素释放激素(LHRH)、促性腺激素释放激素相关肽(GAP)和促黄体生成素(LH)免疫细胞化学的影响
分别饲喂14.2%(标准)和8.1%(蛋白质限制)的饲粮,研究其对生长母羊青春期和促黄体生成素释放激素(LHRH)、促性腺激素相关肽(GAP)、促黄体生成素(LH)激素系统的影响。实验结束时(30 ~ 34周龄),采用免疫细胞化学方法检测下丘脑LHRH、GAP和垂体LH。21周龄时用放射免疫法测定血浆LH。结果表明,降低饲粮蛋白质含量可显著降低21周龄羔羊基础血浆LH浓度。这一组的羊没有一只与标准组的羊同时达到性成熟。然而,蛋白限制羔羊的免疫反应性(ir) LHRH神经元系统发育正常:在视前间隔区、下丘脑和正中隆起(ME)的典型部位,可以很好地看到大量的irLHRH核周、密集的轴突网络和丰富的神经末梢储存物质。LHRH前体的促性腺激素相关肽(GAP)存在于绵羊大脑中含有LHRH的相同神经元群中。营养限制组垂体中lh细胞的比例高3倍。它们表现出肥大和很强的免疫反应。上述结果表明,育成期母羊饲粮中蛋白质缺乏会延缓其青春期发育,但不会影响脑神经细胞中LHRH的合成和加工以及垂体细胞中LH的合成。(摘要删节250字)
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