Stromal and epithelial cells from rat ventral prostate during androgen deprivation and estrogen treatment--regulation of transcription.

M Bacher, U Rausch, H W Goebel, B Polzar, H G Mannherz, G Aumüller
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引用次数: 42

Abstract

To identify the functional capacities of prostatic tissue, the expression of steroid hormone receptors, growth factors, oncogenes and particular enzymes was studied at the RNA level in isolated stromal and epithelial cells of rat ventral prostate (RVP) under different hormonal conditions (androgen deprivation, estrogen treatment). Slot blot and Northern blot analyses of isolated RNA resulted in characteristic changes: In the control prostate, androgen receptor (AR) mRNA was high in epithelium of intact prostate, but low in stroma. Its level was increased after castration in the epithelium during the initial 24 hours, whereas an only slight increase occurred in stroma after one week castration. The AR signal was not altered by estradiol treatment in epithelium and stroma. Conversely, the estrogen receptor (ER) mRNA, predominant in stroma and very low in epithelium, decreased after castration in stroma and epithelium within 24 hours and was absent one week later. After estrogen treatment the ER signal increased considerably in stroma. mRNA of both basic fibroblast growth factor (bFGF) and transforming growth factor beta (TGF-beta) were exclusively found in stroma. Both were low in controls and responded in a different way to castration and estrogen treatment within 24 hours. bFGF was increased in estrogen-treatment animals, while TGF-beta was induced by castration. Shortly after castration (2 hours) v-fos expression increased and reached a maximum after 6 hours, but was no more detectable after 12 hours in epithelium.(ABSTRACT TRUNCATED AT 250 WORDS)

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雄激素剥夺和雌激素治疗期间大鼠腹侧前列腺基质和上皮细胞的转录调控。
为了确定前列腺组织的功能,在RNA水平上研究了不同激素条件(雄激素剥夺和雌激素治疗)下大鼠腹侧前列腺(RVP)离体基质和上皮细胞中类固醇激素受体、生长因子、致癌基因和特定酶的表达。对分离的RNA进行Slot blot和Northern blot分析发现,在对照前列腺中,雄激素受体(AR) mRNA在完整前列腺上皮中表达高,而在间质中表达低。去势后的24小时内,其在上皮细胞中的表达水平升高,而去势一周后,间质细胞中的表达水平仅略有升高。雌二醇未改变上皮和间质细胞的AR信号。相反,雌激素受体(ER) mRNA在间质中占主导地位,上皮中含量极低,去势后24小时内,间质和上皮中雌激素受体mRNA含量下降,1周后消失。雌激素治疗后基质中ER信号明显增加。碱性成纤维细胞生长因子(bFGF)和转化生长因子β (tgf - β) mRNA均在基质中发现。两者在对照中都很低,并且在24小时内对去势和雌激素治疗有不同的反应。雌激素处理动物的bFGF增加,而去势诱导的tgf - β增加。去势后不久(2小时),v-fos表达增加,在6小时后达到最大值,但在12小时后上皮中无法检测到。(摘要删节250字)
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