[The formation and viability of hybrid cells in a population after irradiation at lethal doses].

Radiobiologiia Pub Date : 1993-03-01
G S Kalendo, V V Andrushkevich, O Iu Semeniak, E S Kakpakova
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Abstract

With the use of two genetically labeled lines of Djungarian hamster cells and the method of hybrid selection on a HAT-selective medium it was found that in the irradiated mixed culture of the above cell lines, cells were formed that survived in the conditions of total destruction of irradiated parent cells. The chromosome analysis showed that about 45% of the survived cells were hybrids resulting from the radiation-induced fusion of two initial cell lines. These hybrid cells were capable of reproduction. A subline of hybrid cells was isolated. It is assumed that the radiation-induced process of cell fusion and the formation of viable somatic hybrids might be essential for the survival of cell population in the course of tumor radiotherapy.

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[在致死剂量照射后群体中杂交细胞的形成和生存能力]。
使用两种遗传标记的保加利亚仓鼠细胞系和在hat选择培养基上进行杂交选择的方法发现,在上述细胞系的辐照混合培养中,形成的细胞在受辐照的亲本细胞完全破坏的情况下存活。染色体分析表明,约45%的存活细胞是由辐射诱导的两个初始细胞系融合而成的杂交种。这些杂交细胞能够繁殖。分离出一个杂种细胞亚系。推测在肿瘤放疗过程中,辐射诱导的细胞融合过程和有活力的体细胞杂种的形成可能是细胞群体存活的必要条件。
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[The effect of hematoporphyrin derivative on the postradiation recovery of hemopoiesis in mice]. [The ratio of the contributions from the direct and indirect actions of UV radiation in changing the functional properties of the complement system]. [The effect of irradiation of the thymus, the hypothalamo-hypophyseal area and the gonads on the growth of a transplanted Lewis carcinoma in mice]. [A bacterial model for research on the effect of laser radiation on the intensity of cell division]. [The functional activity of the hypothalamo-hypophyseal-gonadal system in male rats in the late periods after x-ray irradiation].
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