{"title":"Sperm nuclei entering parthenogenetically activated mouse oocytes before the first mitosis transform into pronuclei. An ultrastructural study.","authors":"M Maleszewski","doi":"10.1002/ar.1092430414","DOIUrl":null,"url":null,"abstract":"<p><strong>Background: </strong>This report is an extension of previous observations (Maleszewski 1992. Mol. Reprod. Dev., 33:215-221) on the behavior of mouse sperm nuclei incorporated into parthenogenetically activated mouse oocytes prior to the first cleavage division and undergoing transformation during mitosis.</p><p><strong>Method: </strong>Artificially activated mouse oocytes were inseminated in vitro and an ultrastructural analysis was performed of sperm-derived nuclei present in two parthenogenetic two-cell embryos.</p><p><strong>Results: </strong>Both chromatin and nuclear envelope of sperm derived-nuclei are structurally identical with those of oocyte-derived nuclei and of the nuclei of blastomeres of normal two-cell embryos.</p><p><strong>Conclusions: </strong>Cytoplasm of the parthenogenote during the first mitotic division has the ability to transform sperm nucleus into a male pronucleus just like the cytoplasm of a metaphase II oocyte.</p>","PeriodicalId":50793,"journal":{"name":"Anatomical Record","volume":"243 4","pages":"516-8"},"PeriodicalIF":0.0000,"publicationDate":"1995-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1002/ar.1092430414","citationCount":"7","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Anatomical Record","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1002/ar.1092430414","RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"Agricultural and Biological Sciences","Score":null,"Total":0}
引用次数: 7
Abstract
Background: This report is an extension of previous observations (Maleszewski 1992. Mol. Reprod. Dev., 33:215-221) on the behavior of mouse sperm nuclei incorporated into parthenogenetically activated mouse oocytes prior to the first cleavage division and undergoing transformation during mitosis.
Method: Artificially activated mouse oocytes were inseminated in vitro and an ultrastructural analysis was performed of sperm-derived nuclei present in two parthenogenetic two-cell embryos.
Results: Both chromatin and nuclear envelope of sperm derived-nuclei are structurally identical with those of oocyte-derived nuclei and of the nuclei of blastomeres of normal two-cell embryos.
Conclusions: Cytoplasm of the parthenogenote during the first mitotic division has the ability to transform sperm nucleus into a male pronucleus just like the cytoplasm of a metaphase II oocyte.
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