W W Lorenz, M J Cormier, D J O'Kane, D Hua, A A Escher, A A Szalay
{"title":"Expression of the Renilla reniformis luciferase gene in mammalian cells.","authors":"W W Lorenz, M J Cormier, D J O'Kane, D Hua, A A Escher, A A Szalay","doi":"10.1002/(SICI)1099-1271(199601)11:1<31::AID-BIO398>3.0.CO;2-M","DOIUrl":null,"url":null,"abstract":"<p><p>A cDNA encoding the Renilla reniformis luciferase was expressed in similan and murine cells in a transient and stable manner, respectively. Light emission catalyzed by luciferase was detected from transfected cells both in vitro and in vivo. This work establishes the Renilla luciferase gene as a new efficient marker of gene expression in mammalian cells.</p>","PeriodicalId":15068,"journal":{"name":"Journal of bioluminescence and chemiluminescence","volume":null,"pages":null},"PeriodicalIF":0.0000,"publicationDate":"1996-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"90","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of bioluminescence and chemiluminescence","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1002/(SICI)1099-1271(199601)11:1<31::AID-BIO398>3.0.CO;2-M","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 90
Abstract
A cDNA encoding the Renilla reniformis luciferase was expressed in similan and murine cells in a transient and stable manner, respectively. Light emission catalyzed by luciferase was detected from transfected cells both in vitro and in vivo. This work establishes the Renilla luciferase gene as a new efficient marker of gene expression in mammalian cells.
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