The alkaline comet test on plant cells: A new genotoxicity test for DNA strand breaks in Vicia faba root cells

Mutation Research\/environmental Mutagenesis and Related Subjects Pub Date : 1996-08-08 DOI:10.1016/S0165-1161(96)90017-5

G. Koppen, L. Verschaeve

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引用次数: 150

Abstract

The alkaline comet assay (single cell gel electrophoresis assay) is a sensitive method for the detection of DNA damage. This paper describes the first application of this assay to plant cells for genotoxicological assessment. Germinating Vicia faba (field bean) seedlings were kept in water with either methyl methanesulfonate (MMS), ethyl methanesulfonate (EMS), mitomycin C (MMC), cycloheximide (CH), cadmium chloride (CdCl₂), potassium dichromate (K₂Cr₂O₇), or chromium trichloride (CrCl₃). Nuclei were isolated from the root cells and evaluated for the extent of DNA migration. With the exception of cycloheximide, all agents induced a significant increase in DNA migration. These results indicate that the comet assay may be a valuable tool for monitoring DNA damage in plant systems. However, there was a significant heterogeneity in the extent of DNA migration within and between seedlings, which may be intrinsic to the assay or indicative of sampling problems.

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植物细胞碱性彗星试验:蚕豆根细胞DNA链断裂的一种新的遗传毒性试验

碱性彗星法(单细胞凝胶电泳法)是一种灵敏的DNA损伤检测方法。本文介绍了该方法在植物细胞遗传毒理学评价中的首次应用。将发芽的蚕豆幼苗分别置于含有甲烷磺酸甲酯(MMS)、甲烷磺酸乙酯(EMS)、丝裂霉素C (MMC)、环己亚胺(CH)、氯化镉(CdCl2)、重铬酸钾(K2Cr2O7)或三氯化铬(CrCl3)的水中。从根细胞中分离细胞核并评估DNA迁移的程度。除环己亚胺外，所有药物均能显著增加DNA迁移。这些结果表明，彗星分析可能是监测植物系统DNA损伤的有价值的工具。然而，在幼苗内部和幼苗之间的DNA迁移程度上存在显著的异质性，这可能是化验固有的或表明采样问题。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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$Mutation Research\/environmental Mutagenesis and Related Subjects$

Mutation Research\/environmental Mutagenesis and Related Subjects

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