Differentiation between Mycobacterium tuberculosis and Mycobacterium bovis by a multiplex-polymerase chain reaction.

The Journal of applied bacteriology Pub Date : 1996-06-01 DOI:10.1111/j.1365-2672.1996.tb03263.x

E A Herrera, O Pérez, M Segovia

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引用次数: 20

Abstract

A multiplex-polymerase chain reaction (PCR) assay, based on one-step amplification and detection of three different mycobacterial genomic fragments, was designed for differentiation between Mycobacterium bovis and Mycobacterium tuberculosis. The oligonucleotide primers were chosen from the groEL gene, present in the genus Mycobacterium sp., from the IS6110 insertion sequence, present in Myco. tuberculosis complex and from the mtp40 gene, identified as a specific-species Myco. tuberculosis genomic fragment. This amplification method allowed the detection of two fragments of 576 and 317 base pairs in Myco. bovis and three fragments of 576, 396 and 317 base pairs in Myco. tuberculosis strains, including atypical strains of Myco. tuberculosis where the copy number of the IS6110 element is low. The multiplex-PCR assay described may be a very useful tool for the rapid and specific differentiation of these related mycobacteria and easy to use in medical and veterinary microbiology laboratories.

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多重聚合酶链反应对结核分枝杆菌和牛分枝杆菌的鉴别。

设计了一种基于一步扩增和检测三种不同分枝杆菌基因组片段的多重聚合酶链反应(PCR)方法，用于区分牛分枝杆菌和结核分枝杆菌。寡核苷酸引物取自Myco中IS6110插入序列中的groEL基因，该基因存在于分枝杆菌属。结核复合体并来自mtp40基因，鉴定为Myco特异种。结核基因组片段。该扩增方法可检测Myco中576和317碱基对的两个片段。Myco中576、396和317个碱基对的三个片段。结核菌株，包括非典型结核分枝杆菌菌株。其中IS6110元素的拷贝数较低。所描述的多重pcr检测可能是一种非常有用的工具，可以快速和特异性地区分这些相关的分枝杆菌，并且易于在医学和兽医微生物学实验室中使用。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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The Journal of applied bacteriology

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