Detection of Nitrosococcus oceanus in a Mediterranean lagoon by immunofluorescence.

R Zaccone, G Caruso, M Azzaro
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引用次数: 16

Abstract

The autotrophic ammonium-oxidizing bacterium Nitrosococcus oceanus was studied in the brackish lake of Ganzirri by cultural and immunofluorescent methods. The preparation of the specific antiserum in rabbits is reported. The polyclonal antiserum for Nitrosococcus oceanus cross-reacted with other ammonia oxidizing strains, but not with other marine bacteria. Temporal changes were determined by taking monthly water samples from a station 6 m deep and the distribution in sediments was investigated in four stations. Isolation of a strain of Nitrosococcus sp. was obtained from a sediment sample collected in December. The abundance of Nitrosococcus spp. bacteria correlated positively with particulate organic carbon (POC), particulate organic nitrogen (PON), temperature and total bacteria, whereas there was a negative relationship with oxygen tension. No correlation was found between immunofluorescent and MPN counts of Nitrosococcus spp. bacteria.

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免疫荧光法检测地中海泻湖中海洋亚硝基球菌。
采用培养法和免疫荧光法对甘孜里微咸湖中自养氨氧化细菌海洋亚硝基球菌进行了研究。报道了兔特异性抗血清的制备。海洋亚硝基球菌多克隆抗血清与其他氨氧化菌株发生交叉反应,但与其他海洋细菌无交叉反应。每月从一个6 m深的站点采集水样,确定了时间变化,并调查了四个站点的沉积物分布。从12月采集的沉积物样品中分离出一株亚硝基球菌。亚硝基球菌细菌丰度与颗粒有机碳(POC)、颗粒有机氮(PON)、温度和总菌数呈正相关,与氧张力呈负相关。亚硝基球菌属细菌的免疫荧光计数与MPN计数无相关性。
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