Splenic B-cell activation in lipopolysaccharide-non-responsive C3H/HeJ mice by lipopolysaccharide of Porphyromonas gingivalis.

Experientia Pub Date : 1996-09-15 DOI:10.1007/BF01938879

H Shimauchi, T Ogawa, H Uchida, J Yoshida, H Ogoh, T Nozaki, H Okada

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引用次数: 11

Abstract

Porphyromonas gingivalis 381 lipopolysaccharide (LPS) definitely exhibited mitogenic activity in purified B-cells, separated from spleens of LPS-responsive C3H/HeN mice and LPS-non-responsive C3H/HeJ mice by using a magnetic cell sorting system. The mitogenic activity induced by P. gingivalis LPS was incompletely inhibited by polymyxin B. P. gingivalis LPS also induced a higher production of interleukin-6 (IL-6) in splenic B-cells of C3H/HeN mice as compared with Escherichia coli LPS. Furthermore, P. gingivalis LPS, but not E. coli LPS, induced definite IL-6 production in C3H/HeJ mice. P. gingivalis LPS increased tyrosine, serine/threonine phosphorylation of proteins with various major induced bands in splenic B-cells of both C3H/HeN and C3H/HeJ mice. Additionally, radioiodinated P. gingivalis LPS, similarly to E. coli LPS, bound to a 73-kDa protein on C3H/HeJ as well as C3H/HeN B-cells. Thus P. gingivalis LPS may activate B-cells of C3H/HeJ as well as C3H/HeN mice via the LPS-specific binding protein on the cells.

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牙龈卟啉单胞菌脂多糖对脂多糖无反应C3H/HeJ小鼠脾b细胞的激活作用。

用磁性细胞分选系统从C3H/HeN小鼠和C3H/HeJ小鼠脾脏中分离纯化的b细胞中，牙龈卟啉单胞菌381脂多糖(LPS)明显表现出有丝分裂活性。P. gingivalis LPS诱导的有丝分裂活性被多粘菌素不完全抑制。P. gingivalis LPS诱导的C3H/HeN小鼠脾b细胞中白细胞介素-6 (IL-6)的产生比大肠杆菌LPS高。此外，牙龈卟啉卟啉脂多糖能诱导C3H/HeJ小鼠产生IL-6，而大肠杆菌脂多糖则不能。P. gingivalis LPS增加了C3H/HeN和C3H/HeJ小鼠脾b细胞中各主要诱导带蛋白酪氨酸、丝氨酸/苏氨酸磷酸化。此外，与大肠杆菌LPS类似，放射性碘化的牙龈假单胞杆菌LPS与C3H/HeJ和C3H/HeN b细胞上的73-kDa蛋白结合。因此，P. gingivalis LPS可以通过细胞上LPS特异性结合蛋白激活C3H/HeJ和C3H/HeN小鼠的b细胞。

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