A quantitative cell-ELISA for alpha-galactose specific antibodies in human malaria.

Journal of immunoassay Pub Date : 1996-08-01 DOI:10.1080/01971529608005791

A K Satapathy, B Ravindran

{"title":"A quantitative cell-ELISA for alpha-galactose specific antibodies in human malaria.","authors":"A K Satapathy, B Ravindran","doi":"10.1080/01971529608005791","DOIUrl":null,"url":null,"abstract":"<p><p>Naturally occurring antibodies to alpha-linked galactose (anti- gal) has been reported to be present in large quantities in normal human sera and they seem to play an important role in a variety of infectious as well as autoimmune diseases. A cell-ELISA using glutaraldehyde fixed normal rabbit erythrocytes was developed for quantification of anti-gal in human sera. This assay was compared with three other(commonly used) immunoassays viz. a) agglutination b) enhanced agglutination and c) lipid ELISA-assays for detection of anti-gal in human sera. The cell-ELISA was found to be the most sensitive assay followed by lipid-ELISA, enhanced agglutination and agglutination assay in decreasing order. Anti-gal affinity purified through a column of melibiose-agarose was tested by cell-ELISA. Monolayers of RRBC pre-treated with alpha-galactosidase was not reactive while in monolayers treated with beta-galactosidase, the anti-gal reactivity was comparable to those in untreated RRBC monolayer, thus indicating the high specificity of cell-ELISA for detection of antibodies to alpha-linked galactose.</p>","PeriodicalId":16060,"journal":{"name":"Journal of immunoassay","volume":"17 3","pages":"245-56"},"PeriodicalIF":0.0000,"publicationDate":"1996-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/01971529608005791","citationCount":"2","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of immunoassay","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1080/01971529608005791","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}

引用次数: 2

Abstract

Naturally occurring antibodies to alpha-linked galactose (anti- gal) has been reported to be present in large quantities in normal human sera and they seem to play an important role in a variety of infectious as well as autoimmune diseases. A cell-ELISA using glutaraldehyde fixed normal rabbit erythrocytes was developed for quantification of anti-gal in human sera. This assay was compared with three other(commonly used) immunoassays viz. a) agglutination b) enhanced agglutination and c) lipid ELISA-assays for detection of anti-gal in human sera. The cell-ELISA was found to be the most sensitive assay followed by lipid-ELISA, enhanced agglutination and agglutination assay in decreasing order. Anti-gal affinity purified through a column of melibiose-agarose was tested by cell-ELISA. Monolayers of RRBC pre-treated with alpha-galactosidase was not reactive while in monolayers treated with beta-galactosidase, the anti-gal reactivity was comparable to those in untreated RRBC monolayer, thus indicating the high specificity of cell-ELISA for detection of antibodies to alpha-linked galactose.

查看原文

微信好友朋友圈 QQ好友复制链接

本刊更多论文

人疟疾α -半乳糖特异性抗体的定量细胞elisa检测。

据报道，α -连接半乳糖(抗半乳糖)的天然抗体大量存在于正常人类血清中，它们似乎在各种感染性疾病和自身免疫性疾病中发挥重要作用。建立了用戊二醛固定正常兔红细胞的细胞elisa法定量测定人血清中抗半乳糖的方法。该方法与其他三种常用的免疫测定法进行了比较，即a)凝集法、b)增强凝集法和c)用于检测人血清中抗半乳糖的脂质elisa法。细胞酶联免疫吸附试验最敏感，脂质酶联免疫吸附试验次之，强化凝集试验次之，凝集试验次之。用细胞- elisa法检测通过蜜二糖-琼脂糖柱纯化的抗半乳糖亲和性。经α -半乳糖苷酶预处理的单层RRBC无反应性，而经β -半乳糖苷酶处理的单层RRBC的抗半乳糖反应性与未处理的单层RRBC相当，这表明细胞- elisa检测α -连接半乳糖抗体的特异性很高。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文去求助

来源期刊

Journal of immunoassay

自引率

0.00%

发文量