Long-term behavior of an arterial autograft: a new role for intimal hyperplasia?

J M Bellón, F Jurado, M P De Miguel, B Fraile, J Buján
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引用次数: 7

Abstract

The long-term behavior of an arterial autograft was studied with special attention to the evolution of intimal hyperplasia. An arterial autograft measuring approximately 5 mm in length was implanted in the right common iliac artery of female Sprague-Dawley rats. Animals were sacrificed at 90, 120, 150, 180, 240, 360, 400, 540 and 730 days after implantation. Grafts were evaluated by optical microscopy, electron microscopy, and morphometry. Myointimal cells were marked using an antiactin monoclonal antibody and studied by transmission electron microscopy. In the long term, the myointima of the arterial wall appeared as a consolidated layer formed by smooth muscle cells of contractile phenotype, abundant extracellular material consisting of clumps of elastin and collagen fibers. Cell maturity and degree of differentiation were demonstrated by the incorporation of antiactin antibody. The medial layer of the grafted segment suffered a marked long-term loss of cells and became an acellular layer sustained by the elastic layers. The adventitial layer was markedly cellular and had abundant vasa vasorum. Morphometry showed that the myointimal layer in the operated territory was not uniform and consisted of tongues of varying thickness. The total thickness of the arterial wall did not differ significantly (p > 0.05) between the graft and the proximal and distal areas. The results suggest that the intimal hyperplasia originating during the repair process could assume some functions of the degenerated medial layer, maintaining long-term vascular homeostasis.

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自体动脉移植的长期行为:内膜增生的新作用?
研究了自体动脉移植的长期行为,特别关注内膜增生的演变。将长约5mm的自体动脉移植物植入雌性sd大鼠右侧髂总动脉。分别于植入后90、120、150、180、240、360、400、540、730天处死。通过光学显微镜、电子显微镜和形态计量学对移植物进行评估。用抗肌动蛋白单克隆抗体标记肌内膜细胞,并用透射电镜观察。长期来看,动脉壁肌内膜表现为收缩型平滑肌细胞形成的固结层,细胞外物质丰富,由弹性蛋白和胶原纤维团块组成。结合抗肌动蛋白抗体检测细胞成熟度和分化程度。移植节段内侧层细胞长期损失明显,成为由弹性层支撑的脱细胞层。外皮层细胞明显,有丰富的血管。形态学分析显示手术区域的肌内膜层不均匀,由不同厚度的舌组成。移植物与近端、远端动脉壁总厚度差异无统计学意义(p > 0.05)。结果表明,在修复过程中产生的内膜增生可能承担了退行性内层的某些功能,维持了血管的长期稳态。
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