[Automated blood component collection with the MCS 3p].

Abstract

Objective: Two new protocols for the MCS 3p cellseparator (Haemonetics) for automated collection of blood components were evaluated.

Material and methods: Two units of FFP and one unit of buffy coat free red blood cells (RBC) in additive solution (PAGGS-M) were produced with the D-RBCP protocol. The RBCP-F protocol included additional in-line filtration of the RBC in a closed system. 20 automated blood component collections were performed with each of the two programs. Filtration of the RBC was performed after storage for 24 h at 4 degrees C. Blood cell counts, biochemical characterization of the RBCs (ATP, 2,3-DPG, LDH, HBDH, free Hb, K+, pH) were determined on day 0, 1, 14, 28 and 49.

Results and conclusions: Erythroplasmapheresis with both protocols was performed without any technical problems or adverse reactions. The biochemical parameters of the RBC (until 49 days) were as good or even better as with standard preparations (Hb, 2,3-DPG, ATP, LDH, HBDH, hemolysis, K+). Leukocyte and platelet counts in RBC and FFP (D-RBCP) were well within the range requested by German guidelines. Leukocyte reduction of RBC in RBCP-F was adequate but platelets in FFP exceeded the standard values. The extracorporal volume (771 ml in D-RBCP and 632 ml in RBCP-F) was rather large and at least should be adaptable to the donor's individual blood volume.