Antimutagens from Plumeria acuminata Ait

Abstract

Four isolates, A₁, C₁, D₃, and F₂, from the ethanol extract of the green leaves of Plumeria acuminata Ait. showed antimutagenic activity. The antimutagens were isolated from he bioactive hexane and carbon tetrachloride fractions

Four isolates, A₁, C₁, D₃, and F₂, from the ethanol extract of the green leaves of Plumeria acuminata Ait. showed antimutagenic activity. The antimutagens were isolated from the bioactive hexane and carbon tetrachloride fractions following a bioactivity-directed fractionation scheme and using the micronucleus test to monitor the antimutagenic activities. Structure elucidation studies indicated that C₁ is stigmast-7-enol [1], D₃ is lupeol carboxylic acid [2] and F₂ is ursolic acid [3]. The structure of A₁ was not fully elucidated but MS data suggested that it contained a long hydrocarbon chain. At a dosage of 2 mg isolate/25 g mouse, A₁ reduced the number of micronucleated polychromatic erythrocytes (MPCE) induced by the mutagen, mitomycin C, by 75%, C₁ by 80%, D₃ by 57%, and F₂ by 76%. Compound A₂ was also isolated but was found inactive. Its structure was identified to be lupeol acetate [4].