Annual changes in serum vitellogenin concentrations in viviparous eelpout, Zoarces elongatus

Abstract

Vitellogenin of matrotrophic viviparous eelpout (Zoarces elongatus) was purified from estradiol-17β (E₂) treated immature male sera by gel chromatography and anion exchange chromatography. Isolated vitellogenin has a molecular weight of 540 kDa estimated by gel chromatography. Serum levels of vitellogenin in females were measured during oocyte development and gestation by single radial immunodiffusion. Serum vitellogenin level was low (less than 0.2 mg/ml) during the early vitellogenic period, increased in the late vitellogenic period to a peak level (6.4 ± 2.1 mg/ml) at the beginning of gestation. After that it rapidly decreased to a low level (0.1 ± 0.1 mg/ml) during the early gestation period. Levels of vitellogenin remained low throughout the gestation period. Serum E₂ levels in females showed increased from 1.3 to 3.0 ng/ml during the late vitellogenic period, and declined to 0.4 ng/ml during the early gestation period. Serum levels of E₂ showed good correlation with serum vitellogenin levels, suggesting that the vitellogenin synthesis is controlled by E₂ in this species. These results combined with the matrotrophic growth of embryo during gestation suggest that there is a shift in the synthesis of maternal nutritional products for embryos from the yolk to other nutrients.