Bacterium-assisted invasion of Entamoeba histolytica through human enteric epithelia in two-compartment chambers.

Invasion & metastasis Pub Date : 1997-01-01
A Leroy, G De Bruyne, A Verspeelt, T Lauwaet, H Nelis, M Mareel
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Abstract

Entamoeba histolytica trophozoites initiate amebiasis by invasion into the enteric mucosa. It is the aim of our experiments to understand how bacteria and leukocytes act during amebic invasion through enteric cell layers. Cocultures were established in two-compartment chambers and studied by measurement of transepithelial electrical resistance (TER) and by histological examination. Trophozoites caused a decrease in TER that was followed by formation of holes in the enteric cell layer and transfilter migration of trophozoites. Phagocytosed bacteria activated trophozoites that opened the intracellular junctions and provided access for the invasion of bacteria. Leukocytes had no effect on the different steps of invasion of the trophozoites through the human enteric cell layers. We conclude that trophozoites, eventually assisted by enteric bacteria, disrupt enterocytic tight junctions before they open the enteric cell layer and invade through it.

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细菌辅助溶组织内阿米巴通过人肠上皮在双室室侵袭。
溶组织内阿米巴滋养体通过侵入肠黏膜引发阿米巴病。我们的实验目的是了解细菌和白细胞在阿米巴通过肠细胞层入侵时的作用。共培养在双室室中建立,并通过测量上皮电阻(TER)和组织学检查进行研究。滋养体导致TER降低,随后在肠细胞层形成孔洞,滋养体跨滤迁移。被吞噬的细菌激活滋养体,滋养体打开细胞内连接,为细菌的入侵提供通道。白细胞对滋养体侵入人体肠细胞层的不同步骤没有影响。我们得出结论,滋养体最终在肠细菌的协助下,在它们打开肠细胞层并侵入之前破坏了肠细胞的紧密连接。
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