Purification and cell attachment activity of a D-galactose-binding lectin from the skin of sea hare, Aplysia kurodai.

Y Ozeki
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引用次数: 8

Abstract

A D-galactose-binding lectin which does not require Ca2+ or reducing reagents to induce activity was purified from skin of sea hare, Aplysia kurodai, by affinity chromatography. Skin lectin was confirmed to be a disulfide-bonded heteromultimer with molecular mass of 200 kDa, consisting of 28 kDa and 26 kDa subunits by SDS-PAGE and two-dimensional SDS-PAGE. Human rhabdomyosarcoma cells attached to and spread on plastic plates coated with lectin. Cell adhesion induced by lectin was completely inhibited by the addition of lactose.

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海兔皮肤d -半乳糖结合凝集素的纯化及细胞附着活性研究。
采用亲和层析法从黑黑海兔皮肤中纯化了一种不需要Ca2+或还原试剂诱导活性的d -半乳糖结合凝集素。通过SDS-PAGE和二维SDS-PAGE证实皮肤凝集素为二硫键异聚物,分子量为200 kDa,由28 kDa和26 kDa亚基组成。人横纹肌肉瘤细胞附着并扩散在涂有凝集素的塑料板上。乳糖的加入完全抑制了凝集素诱导的细胞粘附。
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