Effects of eicosapentaenoic acid and its 15-hydroperoxy and 15-hydroxy derivatives on glucosamine synthetase activity in rabbit gastric mucosa.

Biochemistry and molecular biology international Pub Date : 1998-09-01 DOI:10.1080/15216549800203662

T Fujita, S Sakuma, N Yamamoto, Y Fujimoto

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引用次数: 2

Abstract

The effects of eicosapentaenoic acid and its 15-hydroperoxy and 15-hydroxy adducts on the activity of glucosamine synthetase, the rate-limiting enzyme of mucus synthesis, in rabbit gastric antral mucosa were examined. 15-Hydroperoxy-eicosapentaenoic acid inhibited the glucosamine synthetase activity at concentrations of 10, 20 and 50 microM. The effect was concentration-dependent, and the concentration required for 50% inhibition was approximately 20 microM. Eicosapentaenoic acid and its 15-hydroxy adduct had no significant effect on the enzyme activity at the same concentration range. The experiment utilizing Fe2+ revealed that the inhibitory effect of 15-hydroperoxyeicosapentaenoic acid on the glucosamine synthetase activity is not due to hydroxy radical which is expected to be formed from the hydroperoxy adduct. These results suggest that 15-hydroperoxyeicosapentaenoic acid has the potential to reduce the synthesis of gastric mucus by inhibiting the glucosamine synthetase activity.

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二十碳五烯酸及其15-羟基和15-羟基衍生物对兔胃黏膜葡萄糖胺合成酶活性的影响。

研究了二十碳五烯酸及其15-羟基加合物和15-羟基加合物对家兔胃窦黏膜粘液合成限速酶葡萄糖胺合成酶活性的影响。15-氢过氧-二十碳五烯酸在10、20和50 μ m浓度下抑制葡萄糖胺合成酶活性。效果是浓度依赖的，50%抑制所需的浓度约为20微米。在相同浓度范围内，二十碳五烯酸及其15-羟基加合物对酶活性无显著影响。利用Fe2+的实验表明，15-羟基过氧二碳五烯酸对葡萄糖胺合成酶活性的抑制作用不是由于羟基自由基的形成，而羟基自由基是由羟基加合物形成的。这些结果表明，15-氢过氧二碳五烯酸可能通过抑制葡萄糖胺合成酶活性来减少胃粘液的合成。

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Biochemistry and molecular biology international

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