{"title":"The beta subunit of chloroplast ATP synthase (CF0CF1-ATPase) is phosphorylated by casein kinase II.","authors":"M Kanekatsu, H Saito, K Motohashi, T Hisabori","doi":"10.1080/15216549800203602","DOIUrl":null,"url":null,"abstract":"<p><p>We studied a phosphate acceptor for casein kinase II (CK-II) in chloroplasts, and found a 56 kDa protein (p56) as an acceptor, which was partially purified from the stroma of spinach chloroplasts. The N-terminal amino acid sequence of p56 was identical with that of the beta subunit of chloroplast ATP synthase (CF0CF1-ATPase). In addition, the recombinant beta subunit of CF1 was phosphorylated when the subunit was incubated with CK-II. These results suggest that the beta subunit of CF1 is a substrate protein of CK-II in the chloroplast.</p>","PeriodicalId":8770,"journal":{"name":"Biochemistry and molecular biology international","volume":"46 1","pages":"99-105"},"PeriodicalIF":0.0000,"publicationDate":"1998-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/15216549800203602","citationCount":"43","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Biochemistry and molecular biology international","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1080/15216549800203602","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 43
Abstract
We studied a phosphate acceptor for casein kinase II (CK-II) in chloroplasts, and found a 56 kDa protein (p56) as an acceptor, which was partially purified from the stroma of spinach chloroplasts. The N-terminal amino acid sequence of p56 was identical with that of the beta subunit of chloroplast ATP synthase (CF0CF1-ATPase). In addition, the recombinant beta subunit of CF1 was phosphorylated when the subunit was incubated with CK-II. These results suggest that the beta subunit of CF1 is a substrate protein of CK-II in the chloroplast.
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