M Mizuno, K Minato, H Ito, M Kawade, H Terai, H Tsuchida
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引用次数: 126
Abstract
Anti-tumor active polysaccharide against Sarcoma 180 was isolated by DEAE-Sepharose CL-6B and Sepharose 4B column chromatography from the hot-water soluble fraction of the mycelium of liquid-cultured Agaricus blazei mill. This polysaccharide did not react with antibodies of anti-tumor polysaccharides such as lentinan, gliforan, and FIII-2-b which is one of anti-tumor polysaccharides from Agaricus blazei. Moreover, the analyses of 13C-NMR and GC-MS suggested that this polysaccharide was preliminarily glucomannan with a main chain of beta-1,2-linked D-mannopyranosyl residues and beta-D-glucopyranosyl-3-O-beta-D-glucopyranosyl residues as a side chain. This polysaccharide was completely different from the anti-tumor polysaccharide from fruiting body of Agaricus blazei, beta-1,6-glucan.
采用DEAE-Sepharose CL-6B和Sepharose 4B柱层析法从液体培养姬松茸菌丝体的热水可溶性部分分离得到抗肉瘤180的活性多糖。该多糖与香菇多糖、gliforan及姬松茸抗肿瘤多糖FIII-2-b等抗肿瘤多糖的抗体均无反应。13C-NMR和GC-MS分析初步表明该多糖为葡甘露聚糖,主链为β -1,2-连接的d -甘露吡喃基残基,侧链为β - d -葡萄糖吡喃基-3- o - β - d -葡萄糖吡喃基残基。该多糖与姬松茸子实体中的抗肿瘤多糖β -1,6-葡聚糖完全不同。