Tissue-specific posttranscriptional downregulation of expression of the S100A4(mts1) gene in transgenic animals.

N Ambartsumian, J Klingelhöfer, M Grigorian, O Karlstrøm, N Sidenius, G Georgiev, E Lukanidin
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引用次数: 34

Abstract

The S100A4(mts1) is a gene associated with generation of metastatic disease. In order to analyze the consequences of alteration of the pattern of expression of the S100A4(mts1) gene we obtained strains of transgenic mice bearing the S100A4(mts1) gene under the control of a ubiquitous and constitutive 3-hydroxy-3-methylglutaryl CoA reductase (HMGCR) gene promoter. In transgenic animals the expression of the transgene RNA was detected in all organs, but only some of the organs showed elevated levels of the protein. Expression of the S100A4(Mts1) protein was downregulated in the organs that normally do not express the gene in the wild-type animal. The transgene RNA is detected in the polysomes indicating that it could be translated into the S100A4(Mts1) protein. The specificity of the S100A4(Mts1) protein expression is determined by a complex mechanism including regulation of translation and/or posttranslational degradation.

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转基因动物中S100A4(mts1)基因表达的组织特异性转录后下调
S100A4(mts1)是一个与转移性疾病产生相关的基因。为了分析S100A4(mts1)基因表达模式改变的后果,我们在普遍存在的组成型3-羟基-3-甲基戊二酰辅酶a还原酶(HMGCR)基因启动子的控制下获得了携带S100A4(mts1)基因的转基因小鼠菌株。在转基因动物的所有器官中都检测到转基因RNA的表达,但只有部分器官显示出蛋白质水平升高。S100A4(Mts1)蛋白在正常情况下不表达的野生型动物器官中表达下调。在多体中检测到转基因RNA,表明它可以翻译成S100A4(Mts1)蛋白。S100A4(Mts1)蛋白表达的特异性是由包括翻译和/或翻译后降解调控在内的复杂机制决定的。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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