Microelectronic sensors for measurement of electromagnetic fields of living cells and experimental results

František Jelı́nek , Jiřı́ Pokorný , Jaroslav Šaroch , Viktor Trkal , Jiřı́ Hašek , Bohuslav Palán
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引用次数: 33

Abstract

Microelectronic sensors are used for measurements of electromagnetic fields generated by synchronized cultures of yeast cells. Cold sensitive mutant tub2-401 of Saccharomyces cerevisiae is used. The measured electromagnetic signals in the frequency range from 8 to 9 MHz are compared with evolution of the reassembled microtubules. The detected signals peak in the time interval 25–30 min and 45–60 min after the release of the cells from the restrictive to the permissive temperature. The first maximum corresponds to the stage when the mitotic spindle is formed and binds chromatids. The second maximum is measured when the processes of anaphase A and of anaphase B take place.

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用于测量活细胞电磁场的微电子传感器及其实验结果
微电子传感器用于测量酵母细胞同步培养产生的电磁场。使用酿酒酵母的冷敏感突变体tub2-401。将测量到的8 ~ 9 MHz频率范围内的电磁信号与重组微管的演化进行了比较。检测到的信号在细胞从限制温度释放到允许温度后的25-30分钟和45-60分钟的时间间隔内达到峰值。第一个最大值对应于有丝分裂纺锤体形成并结合染色单体的阶段。第二个最大值是在后期A和后期B的过程发生时测量的。
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A bibliometric survey and a citation-based bibliography. Bioelectrochemistry and bioenergetics a bibliometric survey of volumes 1–48 Bioelectrochemistry and bioenergetics cited author index Bioelectrochemistry and bioenergetics citation-based bibliography, 1975–1998 Cumulative indexes of volumes 1-49.
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